Citrate Synthase/CS 蛋白, Human (sf9, His)

Citrate Synthase (CS) is a key enzyme in carbohydrate metabolism, specifically in the tricarboxylic acid (TCA) cycle, where it catalyzes the conversion of oxaloacetate and acetyl-CoA to form citrate. This enzymatic process represents the first and crucial step in the TCA cycle, which is fundamental to cellular energy production. By initiating the cycle and facilitating the condensation of acetyl-CoA with oxaloacetate, CS plays a central role in channeling metabolic intermediates towards the production of energy-rich molecules. This step not only generates citrate but also sets the stage for subsequent reactions in the TCA cycle, ultimately contributing to the efficient extraction of energy from carbohydrates and other metabolic substrates. The activity of CS is pivotal in maintaining cellular energy homeostasis and ensuring the proper functioning of aerobic metabolism. Citrate Synthase/CS Protein, Human (sf9, His) is the recombinant human-derived Citrate Synthase/CS protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of Citrate Synthase/CS Protein, Human (sf9, His) is 439 a.a., with molecular weight of ~46 kDa.

柠檬酸合成酶 (CS) 是碳水化合物代谢中的关键酶，特别是在三羧酸 (TCA) 循环中，它催化草酰乙酸和乙酰辅酶 A 转化为柠檬酸。这种酶促过程代表了 TCA 循环的第一步，也是关键的一步，而 TCA 循环是细胞能量生产的基础。通过启动循环并促进乙酰辅酶A与草酰乙酸的缩合，CS在引导代谢中间体生成富含能量的分子方面发挥着核心作用。这一步骤不仅产生柠檬酸盐，还为 TCA 循环中的后续反应奠定了基础，最终有助于从碳水化合物和其他代谢底物中有效提取能量。 CS 的活性对于维持细胞能量稳态和确保有氧代谢的正常运行至关重要。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

Sf9 insect cells

C-His

O75390 (A28-G466)

1431  [NCBI]

ASSTNLKDILADLIPKEQARIKTFRQQHGKTVVGQITVDMMYGGMRGMKGLVYETSVLDPDEGIRFRGFSIPECQKLLPKAKGGEEPLPEGLFWLLVTGHIPTEEQVSWLSKEWAKRAALPSHVVTMLDNFPTNLHPMSQLSAAVTALNSESNFARAYAQGISRTKYWELIYEDSMDLIAKLPCVAAKIYRNLYREGSGIGAIDSNLDWSHNFTNMLGYTDHQFTELTRLYLTIHSDHEGGNVSAHTSHLVGSALSDPYLSFAAAMNGLAGPLHGLANQEVLVWLTQLQKEVGKDVSDEKLRDYIWNTLNSGRVVPGYGHAVLRKTDPRYTCQREFALKHLPNDPMFKLVAQLYKIVPNVLLEQGKAKNPWPNVDAHSGVLLQYYGMTEMNYYTVLFGVSRALGVLAQLIWSRALGFPLERPKSMSTEGLMKFVDSKSG

Approximately 46 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, 10% Glycerol, pH 7.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.