CK2 alpha/CSNK2A1 蛋白, Human (sf9, GST)

CK2α/CSNK2A1 protein can phosphorylate serine or threonine. It is involved in the regulation of cell cycle, apoptosis, transcription and viral infection, and has protective effects on the spindle assembly checkpoint and p53/TP53-mediated apoptosis. CK2α/CSNK2A1 can also participate in signal transduction by phosphorylating various factors, affecting circadian clock function and virus life cycle. CK2 alpha/CSNK2A1 Protein, Human (sf9, GST) is the recombinant human-derived CK2 alpha/CSNK2A1 protein, expressed by Sf9 insect cells , with N-GST labeled tag. The total length of CK2 alpha/CSNK2A1 Protein, Human (sf9, GST) is 391 a.a., with molecular weight of ~65 KDa.

CSNK2A2 蛋白的催化亚基作为组成型活性丝氨酸/苏氨酸蛋白激酶复合物的核心成分发挥作用，对磷酸化丝氨酸或苏氨酸 C 端酸性残基表现出广泛的底物特异性。CSNK2A2 作为调节节点，在多种细胞过程中发挥着关键作用，包括细胞周期进程、细胞凋亡、转录和病毒感染。它有助于有丝分裂期间 p53/TP53 依赖性纺锤体组装检查点，维持细胞周期蛋白-B-CDK1 活性和响应纺锤体损伤的 G2 停滞。此外，CSNK2A2 还参与细胞凋亡调节，磷酸化半胱天冬酶和 NOL3 等关键参与者。其影响延伸至转录调控，通过 RNA 聚合酶和众多转录因子的直接磷酸化。在复杂的细胞信号传导过程中，CSNK2A2 参与 Wnt 信号传导，充当细胞外蛋白的胞外激酶，并使参与不同病毒生命周期的各种蛋白磷酸化。此外，它在生物钟功能中发挥着至关重要的作用，并通过磷酸化 FMR1 有助于形成无膜区室。总体而言，CSNK2A2 作为细胞对不同刺激做出反应的中心协调者而出现，展示了其在维持细胞稳态方面的重要性。

The specific activity was determined to be 38 nmol/min/mg using casein as substrate.

Human

Sf9 insect cells

N-GST

P68400-1 (M1-Q391)

1457  [NCBI]

Approximately 65 kDa.

Greater than 90% as determined by reducing SDS-PAGE

溶液.

Supplied as a 0.2 μm filtered solution of 50 mM Tris, 100 mM NaCl, 0.5 mM PMSF, 0.5 mM GSH, pH 8.0.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.