CMBL 蛋白, Human (His)

The CMBL protein is a cysteine hydrolase that converts olmesartan medoxomil to its active form olmesartan, an angiotensin receptor blocker. It may also activate beta-lactam antibiotics such as faropenem etexil and renamcillin. CMBL Protein, Human (His) is the recombinant human-derived CMBL protein, expressed by E. coli , with N-His labeled tag. The total length of CMBL Protein, Human (His) is 245 a.a., with molecular weight of ~28 kDa.

CMBL 蛋白起到半胱氨酸水解酶的作用，表明能够将前药 olmesartan medoxomil 转化为其活性代谢物 olmerstatan（一种血管紧张素受体阻滞剂），主要在肝脏和肠道内进行。此外，CMBL 可能在 β-内酰胺抗生素（如法罗培南酯和仑氨西林）的激活中发挥作用。其酶活性被对氯汞苯甲酸酯 (PCMB) 强烈抑制，并被双对硝基苯磷酸盐 (BNPP) 部分抑制。值得注意的是，CMBL 不受 DFP、PMSF、eserine 或 EDTA 等抑制剂的影响。

1.The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.
2.Measured by its ability to inhibit the proliferation of human colon cancer cells. The ED₅₀ for this effect is 38.07 ng/mL. Corresponding to a specific activity is 2.627×10⁴ Unit/mg

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  Measured by its ability to inhibit the proliferation of human colon cancer cells. The ED₅₀ for this effect is 38.07 ng/mL. Corresponding to a specific activity is 2.627×10⁴ Unit/mg.

Human

E. coli

N-His

Q96DG6 (M1-M245)

134147  [NCBI]

MANEAYPCPCDIGHRLEYGGLGREVQVEHIKAYVTKSPVDAGKAVIVIQDIFGWQLPNTRYIADMISGNGYTTIVPDFFVGQEPWDPSGDWSIFPEWLKTRNAQKIDREISAILKYLKQQCHAQKIGIVGFCWGGTAVHHLMMKYSEFRAGVSVYGIVKDSEDIYNLKNPTLFIFAENDVVIPLKDVSLLTQKLKEHCKVEYQIKTFSGQTHGFVHRKREDCSPADKPYIDEARRNLIEWLNKYM

Approximately 28 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 0.1% Brij35, pH 8.0 or 50 mM Tris-HCL, 300 mM NaCl, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.