CNTN5/Contactin-5 蛋白, Mouse (HEK293, His)

The CNTN5/Contactin-5 protein is a member of the contactin family and plays a crucial role in mediating cell surface interactions during nervous system development. Although it exhibits neurite growth-promoting activity in cerebral cortical neurons, it does not exhibit the same effect in hippocampal neurons, highlighting its cell type-specific function in neuronal growth. CNTN5/Contactin-5 Protein, Mouse (HEK293, His) is the recombinant mouse-derived CNTN5/Contactin-5 protein, expressed by HEK293 , with C-His labeled tag.

Contactin-5 (CNTN5) 是一种在神经系统发育过程中介导细胞表面相互作用至关重要的蛋白质，强调了它在神经连接建立中的作用。它表现出神经突生长促进活性，特别是在大脑皮层神经元中，突出显示它参与大脑发育过程中神经元过程的延伸（通过相似性）。有趣的是，在海马神经元中没有观察到这种神经突生长促进活性，这表明其功能依赖于环境。CNTN5 还与听觉系统内的神经元活动有关，突显了其在神经系统各个区域中的不同作用。此外，CNTN5 与 PTPRG 相互作用，表明其可能参与神经发育过程中的信号通路或细胞粘附。Contactin-5 的多方面功能和相互作用强调了它在神经系统发育过程中协调复杂过程的重要性。

Measured by the ability of the immobilized protein to support the adhesion of C6 Rat brain glial cells. When 5×10⁴ cells/well are added to CNTN5 coated plates (2.5μg/mL and 100 μL/well), approximately 51.30% will adhere specifically after 60 minutes at 37°C.

Mouse

HEK293

C-10*His

P68500 (L24-Q1058)

244682  [NCBI]

LSGLSTSYAALLRIKKSSTSPFGSKSRPRFSSPSLGTISVSPPSWRGAAQQYHSPGNLYHSSDAFRQDESVDYGPVFVQEPDDVIFPTDSDEKKVALNCEVRGNPSPSYRWLRNGTEIALESDYRYSLIDGTFIISNPSELRDSGLYQCLATNSFGSILSREATLQFAYLGNFSGRTRSAVSVREGQGVVLMCSPPPHSPEIIYSWVFNEFPSFVAEDSRRFISQETGNLYISKVQTSDVGSYICLVKNAVTNARVLSPPTPLTLRNDGVMGEYEPKIEVHFPFTVTAAKGTTVKMECFALGNPVPTITWMKVNGYIPSKSRLRKSQAVLEIPNLQLDDAGIYECTAENSRGKNSFRGQLQIFTYPHWVQKLNDTQLDSGSPLQWECKATGKPRPTYRWLKNGAPLLPQSRVDTVNGILAIQSVNQSDAGMYQCLAENKYGAIYASAELKILASPPSFELNQVKKSIIVTKDRGVLIECEPQGSPKPAISWRKGDKAVRANKRIAILPDGSLRILNASKADEGKYICQGVNIFGSAEIIASLSVKEPTRIELTPKRTELTVGESIVLNCKAIHDASLDVTFYWTLKGQPIDFEKEGGHFENIRAQASSADLMIRNILLMHAGRYGCRVQTTADSVSDEAELLVRGPPGPPGVVIVEEITESTATLSWSPATDNHSPISSYNLQARSPFSLGWQTVKTVPEVITGDMESAMAVDLNPWVEYEFRVVATNPIGTGDPSIPSRMIRTNEAVPKTAPSNVSGRSGRRHELVIAWEPVSEEFQNGEGFGYIVAFRPNGTRGWKEKMVTSSEASKFIYRDESVPPLTPFEVKVGVYNNKGDGPFSQIVVICSAEGEPTAAPTDVTATSVSVSEIFVVWKHVKESLGRPQGFEISYWKDTEPEDSVETVRTRGNESFVMLTGLEGNTLYHLTVRAYNGAGYGPPSREASTTTKRHPPREPPGNLRWEQQGSQVSLGWEPVRPLANESEVMGYKVFYRQEGHSEGQVIETQKPQAVVPLPEAGVYIIEVRAYSEGGDGTASSQ

Approximately 110-135 kDa due to the glycosylation.

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.