CNTN6/Contactin-6 蛋白, Human (HEK293, Fc)

The Contactin-6 (CNTN6) protein is a key player in nervous system development, promoting critical cell surface interactions. It actively participates in oligodendrocyte generation by acting as a ligand for NOTCH1. CNTN6/Contactin-6 Protein, Human (HEK293, Fc) is the recombinant human-derived CNTN6/Contactin-6 protein, expressed by HEK293 , with C-hFc labeled tag.

Contactin-6 (CNTN6) 是神经系统发育的关键参与者，其中 contactins 介导细胞表面相互作用。CNTN6 通过作为 NOTCH1 的配体参与少突胶质细胞的生成。它与 NOTCH1 的结合导致通过释放 notch 胞内结构域 (NICD) 来激活 NOTCH1，从而促进其易位至细胞核。这种对 NOTCH1 信号传导的参与强调了 CNTN6 在调节与少突胶质细胞发育相关的细胞过程中的作用。此外，CNTN6 与运动协调有关（通过相似性），表明它对控制运动功能的神经回路有潜在贡献。与 PTPRG 的相互作用进一步强调了 CNTN6 在神经系统发育过程中参与的复杂调控网络。

Measured by the ability of the immobilized protein to support the adhesion of Neuro-2A mouse neuroblastoma cells. The ED₅₀ for this effect is 1.535 µg/mL, corresponding to a specific activity is 651.46 units/mg.

Human

HEK293

C-hFc

Q9UQ52/NP_055276.1 (D20-S999)

27255  [NCBI]

DGLLSRPIFTQEPHDVIFPLDLSKSEVILNCAANGYPSPHYRWKQNGTDIDFTMSYHYRLDGGSLAINSPHTDQDIGMYQCLATNLLGTILSRKAKLQFAYIEDFETKTRSTVSVREGQGVVLLCGPPPHFGDLSYAWTFNDNPLYVQEDNRRFVSQETGNLYIAKVEPSDVGNYTCFITNKEAQRSVQGPPTPLVQRTDGVMGEYEPKIEVRFPETIQAAKDSSVKLECFALGNPVPDISWRRLDGSPLPGKVKYSKSQAILEIPNFQQEDEGFYECIASNLRGRNLAKGQLIFYAPPEWEQKIQNTHLSIYDNLLWECKASGKPNPWYTWLKNGERLNPEERIQIENGTLIITMLNVSDSGVYQCAAENKYQIIYANAELRVLASAPDFSKSPVKKKSFVQVGGDIVIGCKPNAFPRAAISWKRGTETLRQSKRIFLLEDGSLKIYNITRSDAGSYTCIATNQFGTAKNTGSLIVKERTVITVPPSKMDVTVGESIVLPCQVSHDPSIEVVFVWFFNGDVIDLKKGVAHFERIGGESVGDLMIRNIQLHHSGKYLCTVQTTLESLSAVADIIVRGPPGPPEDVQVEDISSTTSQLSWRAGPDNNSPIQIFTIQTRTPFSVGWQAVATVPEILNGKTYNATVVGLSPWVEYEFRVVAGNSIGIGEPSEPSELLRTKASVPVVAPVNIHGGGGSRSELVITWESIPEELQNGEGFGYIIMFRPVGSTTWSKEKVSSVESSRFVYRNESIIPLSPFEVKVGVYNNEGEGSLSTVTIVYSGEDEPQLAPRGTSLQSFSASEMEVSWNAIAWNRNTGRVLGYEVLYWTDDSKESMIGKIRVSGNVTTKNITGLKANTIYFASVRAYNTAGTGPSSPPVNVTTKKSPPSQPPANIAWKLTNSKLCLNWEHVKTMENESEVLGYKILYRQNRQSKTHILETNNTSAELLVPFEEDYLIEIRTVSDGGDGSSSEEIRIPKMSSLSS

Approximately 125-180 kDa due to the glycosylation.

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4, 5% trehalose, 5% mannitol and 0.01% Tween80.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.