1. 重组蛋白
  2. Enzymes & Regulators
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  4. Coagulation Factor III/F3 蛋白, Canine (HEK293, His)

Coagulation Factor III/F3 蛋白, Canine (HEK293, His)

目录号: HY-P700695
产品使用指南

凝血因子 III (F3) 或组织因子 (TF) 通过与循环因子 VII 或 VIIa 形成复合物来启动血液凝固。[TF:VIIa] 复合物通过有限的蛋白水解作用关键性地激活因子 IX 或 X,启动细胞表面的凝血级联反应。F3 与 HSPE 的相互作用(受肝素抑制)促进了活化因子 X 的产生,强调了其在调节止血过程中的核心作用。Coagulation Factor III/F3 蛋白, Canine (HEK293, His) 是重组的 凝血因子 III/F3 蛋白,由 HEK293 表达,带有 C-His 标签。Coagulation Factor III/F3 蛋白, Canine (HEK293, His) 全长 214 个氨基酸,分子量为 35-45 kDa。

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描述

Coagulation Factor III (F3), or Tissue Factor (TF), initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex critically activates factors IX or X through limited proteolysis, initiating the coagulation cascade on the cell surface. F3's interaction with HSPE, inhibited by heparin, promotes the generation of activated factor X, emphasizing its central role in regulating hemostatic processes. Coagulation Factor III/F3 Protein, Canine (HEK293, His) is the recombinant canine-derived Coagulation Factor III/F3 protein, expressed by HEK293 , with C-His labeled tag. The total length of Coagulation Factor III/F3 Protein, Canine (HEK293, His) is 214 a.a., with molecular weight of 35-45 kDa.

研究背景

凝血因子 III (F3),也称为组织因子 (TF),通过与循环因子 VII 或 VIIa 形成复合物,充当血液凝固的关键引发剂。这种 [TF:VIIa] 复合物在通过特定的有限蛋白水解激活因子 IX 或 X 中发挥着关键作用,从而启动细胞表面凝固蛋白酶级联的组装和传播。值得注意的是,F3 与 HSPE 相互作用,而肝素会抑制这种相互作用。F3 和 HSPE 之间的相互作用促进了活化因子 X 的生成,并在活化因子 VII 存在的情况下激活凝血,强调了 F3 在止血过程调节中的核心参与。

种属

Canine

表达系统

HEK293

标签

C-His

蛋白编号

Q4W6L5 (T35-E248)

基因 ID
蛋白结构
N-term
F3 (T35-E248)
Accession # Q4W6L5
His
C-term
同用名
Tissue factor; Coagulation Factor III; TF; Thromboplastin; CD142; F3; FLJ17960; TFA
分子量

35-45 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

Coagulation Factor III/F3 Protein, Canine (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Coagulation Factor III/F3 Protein, Canine (HEK293, His)
目录号:
HY-P700695
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