Coagulation factor IX/F9 蛋白, Human (HEK293, His)

Factor IX (F9) is a vitamin K-dependent plasma protein that plays a critical role in the intrinsic pathway of coagulation. Factor IX coordinates the conversion of factor X to its active form in the presence of Ca(2+) ions, phospholipids, and factor VIIIa. Coagulation factor IX/F9 Protein, Human (HEK293, His) is the recombinant human-derived Coagulation factor IX/F9 protein, expressed by HEK293 , with C-6*His labeled tag.

凝血因子 IX，也称为 F9，是一种维生素 K 依赖性血浆蛋白，对于血液凝固至关重要，尤其是在内源性途径中。在 Ca(2+) 离子、磷脂和因子 VIIIa 存在的情况下，该蛋白质在将因子 X 转化为其活性形式方面发挥着关键作用。作为凝血级联的关键组成部分，因子 IX 有助于形成稳定的血凝块，防止过度出血。所描述的机制强调了因子 IX 与其他凝血因子和辅助因子之间复杂的相互作用，强调了其在维持止血中的重要作用。

1. Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2. Read at excitation and emission wavelengths of 320 nm and 405 nm (top read).The specific activity is >200 pmol/min/μg, as measured under the described conditions.
2. Measured by its ability to cleave the peptide substrate Z-D-Arg-Gly-Arg-pNA and the specific activity is >20 pmol/min/ug. (Activate human Coagulation factor IX wih Thermolysin in Assay Buffer, 37°C, 90 min.)

Human

HEK293

C-6*His

P00740-1 (T29-T461)

2158  [NCBI]

TVFLDHENANKILNRPKRYNSGKLEEFVQGNLERECMEEKCSFEEAREVFENTERTTEFWKQYVDGDQCESNPCLNGGSCKDDINSYECWCPFGFEGKNCELDVTCNIKNGRCEQFCKNSADNKVVCSCTEGYRLAENQKSCEPAVPFPCGRVSVSQTSKLTRAETVFPDVDYVNSTEAETILDNITQSTQSFNDFTRVVGGEDAKPGQFPWQVVLNGKVDAFCGGSIVNEKWIVTAAHCVETGVKITVVAGEHNIEETEHTEQKRNVIRIIPHHNYNAAINKYNHDIALLELDEPLVLNSYVTPICIADKEYTNIFLKFGSGYVSGWGRVFHKGRSALVLQYLRVPLVDRATCLRSTKFTIYNNMFCAGFHEGGRDSCQGDSGGPHVTEVEGTSFLTGIISWGEECAMKGKYGIYTKVSRYVNWIKEKTKLT

60-90 kDa

Greater than 95% as determined by reducing SDS-PAGE.

溶液

Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, 10% Glycerol, pH 8.0 or PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.