Complement C3/C3a 蛋白, Bovine (His-SUMO)

Complement C3/C3a proteins play a key role in initiating the complement system, serving as substrates for C3 convertase in both the classical and alternative pathways. Upon activation, C3 undergoes proteolytic cleavage, yielding C3b, which is covalently bound to surface or immune aggregates. Complement C3/C3a Protein, Bovine (His-SUMO) is the recombinant bovine-derived Complement C3/C3a protein, expressed by E. coli , with N-His, N-SUMO labeled tag.

C3 在补体系统的启动中发挥着关键作用，在经典途径和替代途径中充当 C3 转化酶的底物。激活后，C3 经历蛋白水解裂解，生成 C3b，C3b 可以通过其反应性硫酯部分共价结合到细胞表面碳水化合物或免疫聚集体。同时，C3 降解会产生过敏毒素 C3a，在局部炎症过程中充当介质。C3a 诱导平滑肌收缩，增强血管通透性，并刺激肥大细胞和嗜碱性白细胞释放组胺。此外，在慢性炎症中，C3a 充当中性粒细胞的化学引诱剂。

Bovine

E. coli

N-His;N-SUMO

Q2UVX4 (S747-Q953)

280677  [NCBI]

SDLDDDIIPEEDIISRSQFPESWLWTVIEDLKQADKNGISTKLMNVFLKDSITTWEILAVSLSDKKGICVADPYEVTVMQDFFIDLRLPYSVVRNEQVEIRAILYNYREAENLKVRVELLYNPAFCSLATAKKRHQQTITIPARSSVAVPYVIVPLKIGLHEVEVKAAVYNHFISDGVKKTLKVVPEGVRVNKTVAVRTLNPEHLGQ

Approximately 44 kDa.The reducing (R) protein migrat es as 44 kDa in SDS-PAGE may be due to relative charge.

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized after extensive dialysis against solution in 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.