Complement C5 蛋白, Human (HEK293, His)

Complement C5 is activated by C5 convertase, inducing C5-C9 assembly to form the membrane attack complex. The transient C6 binding site on C5b is critical for the formation of the cleavage complex. Complement C5 Protein, Human (HEK293, His) is the recombinant human-derived Complement C5 protein, expressed by HEK293 , with C-His labeled tag. The total length of Complement C5 Protein, Human (HEK293, His) is 1658 a.a., with molecular weight of 110-114 kDa (α chain) & 69-73 kDa (β chain), respectively.

被 C5 转化酶激活后，补体 C5 启动后期补体成分 C5-C9 的自发组装，形成膜攻击复合物。C6 在 C5b 上的瞬时结合位点对于裂解复合物的基础至关重要。补体 C5 的蛋白水解降解产生 C5a 过敏毒素，它是局部炎症过程的介质。C5a 与其受体 C5AR1 相互作用，引发多种反应，例如细胞内钙释放、平滑肌收缩、血管通透性增加以及肥大细胞和嗜碱性白细胞释放组胺。作为一种有效的趋化因子，C5a 刺激多形核白细胞的运动并引导它们向炎症部位迁移，从而有助于协调免疫反应。

Immobilized Human Complement C5, His Tag at 1 μg/mL (100 μl/well) on the plate. Dose response curve for Anti-Complement C5 Antibody, hFc Tag with the EC₅₀ of ≤21.4 ng/mL determined by ELISA.

Human

HEK293

C-His

P01031 (Q19-C1676)

727  [NCBI]

110-114 kDa (α chain) & 69-73 kDa (β chain)

Greater than 95% as determined by Tris-Bis PAGE.

无菌粉末

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 8% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.