Complement C5/C5a 蛋白, Mouse (His)

Complement C5/C5a Protein, Mouse is a recombinant mouse complement component 5a (C5a). C5a is a potent pro-inflammatory mediator and acts as an essential part of the innate immune response^[1].

补体成分 5a (C5a) 是一种由 74 个氨基酸组成的糖蛋白，是一种有效的促炎介质，在补体级联激活后，可通过酶从其前体 C5 上裂解^[1]。

Measured by its ability to induce N-acetyl-beta-D-glucosaminidase release from differentiated U937 human histiocytic lymphoma cells. The ED₅₀ for this effect is 6.447 ng/mL, corresponding to a specific activity is 1.551×10⁵ units/mg.

Mouse

E. coli

N-6*His

P06684 (N679-R755)

15139

NLHLLRQKIEEQAAKYKHSVPKKCCYDGARVNFYETCEERVARVTIGPLCIRAFNECCTIANKIRKESPHKPVQLGR

Approximately 11.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized after extensive dialysis against PBS, pH 7.4, 10% trehalose, 0.02% Tween80.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.