1. 重组蛋白
  2. Receptor Proteins
  3. Cell Adhesion Molecules (CAMs)
  4. Immunoglobulin-like Cell Adhesion Molecules
  5. Contactin-3
  6. Contactin-3/CNTN3 蛋白, Mouse (HEK293, His)

Contactin-3/CNTN3 蛋白, Mouse (HEK293, His)

目录号: HY-P74228
COA 产品使用指南

Contactin-3/CNTN3 蛋白促进神经系统发育中的细胞表面相互作用,促进神经突生长并与 PTPRG 相互作用以调节神经元生长和连接。Contactin-3/CNTN3 蛋白, Mouse (HEK293, His) 是重组的 Contactin-3/CNTN3 蛋白,由 HEK293 表达,带有 C-His 标签。

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规格 价格 是否有货 数量
Free Sample   Apply now  
5 μg ¥670 In-stock
10 μg ¥1080 In-stock
50 μg ¥2800 In-stock
100 μg   询价  

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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

Contactin-3/CNTN3 protein facilitates cell surface interactions in nervous system development, promoting neurite outgrowth and interacting with PTPRG to regulate neuronal growth and connectivity.Contactin-3/CNTN3 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Contactin-3/CNTN3 protein, expressed by HEK293 , with C-His labeled tag.

研究背景

Contactin-3/CNTN3 蛋白在神经系统发育过程中促进细胞表面相互作用方面发挥着至关重要的作用。它表现出促进神经突生长的活性,并与 PTPRG 相互作用,表明它参与调节神经元生长和连接的信号通路。

生物活性

Measured by its binding ability in a functional ELISA. When Recombinant Mouse CNTN3 is present at 2 μg/mL, can bind Recombinant Human Amyloid Precursor. The ED50 for this effect is 0.097 μg/mL.

  • Measured by its binding ability in a functional ELISA. When Recombinant Mouse CNTN3 is present at 2 μg/mL, can bind Recombinant Human Amyloid Precursor. The ED50 for this effect is 0.097 μg/mL.
种属

Mouse

表达系统

HEK293

标签

C-10*His

蛋白编号

Q07409 (E20-G1001)

基因 ID
蛋白结构
N-term
CNTN3 (M1-G1001)
Accession # Q07409
His
C-term
同用名
Contactin-3; BIG-1; CNTN3; KIAA1496; PANG
氨基酸序列

ELLLQGPVFIKEPSNSIFPVDSEDKKITLNCEARGNPSPHYRWQLNGSDIDTSLDHRYKLNGGNLIVINPNRNWDTGSYQCFATNSLGTIVSREAKLQFAYLENFKTRMRSTVSVREGQGVVLLCGPPPHSGELSYAWVFNEYPSFVEEDSRRFVSQETGHLYIAKVEPSDVGNYTCVVTSTVTNTRVLGSPTPLVLRSDGVMGEYEPKIEVQFPETLPAAKGSTVRLECFALGNPVPQINWRRSDGMPFPNKIKLRKFNGMLEIQNFQQEDTGSYECIAENSRGKNVARGRLTYYAKPYWLQLLRDVEIAVEDSLYWECRASGKPKPSYRWLKNGDALVLEERIQIENGALTITNLNVTDSGMFQCIAENKHGLIYSSAELKVVASAPDFSRNPMKKMVQVQVGSLVILDCKPRASPRALSFWKKGDMMVREQARVSFLNDGGLKIMNVTKADAGTYTCTAENQFGKANGTTHLVVTEPTRIILAPSNMDVAVGESVILPCQVQHDPLLDIMFAWYFNGALTDFKKDGSHFEKVGGSSSGDLMIRNIQLKHSGKYVCMVQTGVDSVSSAAELIVRGSPGPPENVKVDEITDTTAQLSWTEGTDSHSPVISYAVQARTPFSVGWQSVRTVPEVIDGKTHTATVVELNPWVEYEFRIVASNKIGGGEPSLPSEKVRTEEAAPEIAPSEVSGGGGSRSELVITWDPVPEELQNGGGFGYVVAFRPLGVTTWIQTVVTSPDNPRYVFRNESIVPFSPYEVKVGVYNNKGEGPFSPVTTVFSAEEEPTVAPSHISAHSLSSSEIEVSWNTIPWKLSNGHLLGYEVRYWNNGGEEESSRKVKVAGNQTSAVLRGLKSNLAYYTAVRAYNSAGAGPFSATVNATTKKTPPSQPPGNVVWNATDTKVLLNWEQVKAMENESEVTGYKVFYRTSSQNNVHVLNTNKTSAELLLPIKEDYIIEVKATTDGGDGTSSEQIRIPRITSMDARG

分子量

Approximately 130-160 kDa due to the glycosylation.

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Contactin-3/CNTN3 Protein, Mouse (HEK293, His)
目录号:
HY-P74228
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