COX-2 蛋白, Human (sf9, His)

COX-2 protein is involved in the alternative pathway of prostaglandin biosynthesis. COX-2 Protein, Human (sf9, His) is the recombinant human-derived COX-2 protein, expressed by Sf9 insect cells , with C-10*His labeled tag.

COX-2 蛋白在前列腺素生物合成途径中充当双重功能酶，前列腺素是炎症反应的关键介质。COX-2 同时充当环氧合酶和过氧化物酶，将花生四烯酸 (AA) 转化为氢过氧内过氧化物前列腺素 G2 (PGG2)，并进一步将其还原为羟基内过氧化物前列腺素 H2 (PGH2)。该催化过程涉及碳 13 处的氢提取，然后插入分子氧以形成前列腺素中特有的内过氧化物桥。COX-2 将其酶活性扩展到二高γ-亚油酸 (DGLA) 和二十碳五烯酸 (EPA)，生成 PGH1 和 PGH3 作为 1 系列和 3 系列前列腺素的前体。此外，COX-2 有助于替代前列腺素生物合成途径，转化 2-花生四烯酰溶血磷脂并代谢 2-花生四烯酰甘油。该酶还在分解相相互作用产物的生成中发挥作用，有助于在炎症过程中合成专门的促分解介质，例如分解素。这种多方面的功能凸显了 COX-2 在前列腺素生成和炎症信号传导的复杂调节中的关键作用。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

Sf9 insect cells

C-10*His

P35354/NP_000954.1 (A18-L604)

5743  [NCBI]

Approximately 66 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris, 100 mM NaCl, 0.5 mM PMSF, 10% Glycerol, pH 8.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.