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  3. CSTL1 蛋白, Human (HEK293, Fc)

CSTL1 蛋白, Human (HEK293, Fc)

目录号: HY-P77344
COA 产品使用指南

半胱氨酸蛋白酶抑制剂样蛋白 1 (CSTL1) 是半胱氨酸蛋白酶抑制剂家族的成员。CSTL1 蛋白, Human (HEK293, Fc) 是重组的 CSTL1 蛋白,由 HEK293 表达,带有 C-hFc 标签。

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描述

Cystatin-like protein 1 (CSTL1) is a member of the cystatin family. CSTL1 Protein, Human (HEK293, Fc) is the recombinant human-derived CSTL1 protein, expressed by HEK293 , with C-hFc labeled tag.

研究背景

CSTL1 蛋白是一种 2 型半胱氨酸蛋白酶抑制剂样蛋白。2 型胱抑素蛋白是一类半胱氨酸蛋白酶抑制剂,存在于多种人体体液和分泌物中。该蛋白质的具体功能尚未确定。半胱氨酸蛋白酶抑制剂超家族包括含有多个半胱氨酸蛋白酶抑制剂样序列的蛋白质。超家族中有3个抑制家族,包括1型半胱氨酸蛋白酶抑制剂(stefins)、2型半胱氨酸蛋白酶抑制剂和激肽原。

种属

Human

表达系统

HEK293

标签

C-hFc

蛋白编号

Q9H114 (A20-R145)

基因 ID

128817  [NCBI]

蛋白结构
N-term
CSTL1 (A20-R145)
Accession # Q9H114
hFc
C-term
同用名
Cystatin-like 1; RCET11; CSTL1
氨基酸序列

AKLGHFQRWEGFQQKLMSKKNMNSTLNFFIQSYNNASNDTYLYRVQRLIRSQMQLTTGVEYIVTVKIGWTKCKRNDTSNSSCPLQSKKLRKSLICESLIYTMPWINYFQLWNNSCLEAEHVGRNLR

分子量

Approximately 45-80 kDa due to the glycosylation

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

CSTL1 Protein, Human (HEK293, Fc) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
CSTL1 Protein, Human (HEK293, Fc)
目录号:
HY-P77344
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