CTHRC1 蛋白, Human (HEK293, His)

CTHRC1 Protein, Human (HEK293, His) is a negative regulator of collagen matrix deposition. CTHRC1, a secreted 28-kDa protein, is a glycosylated protein with a signal sequence.

CTHRC1 是一种糖基化的分泌型 28 kDa 蛋白，从低等脊索动物到哺乳动物都高度保守。具有 12 个 Gly-XY 重复序列的短胶原基序似乎负责蛋白质的三聚化，这使得该分子容易被胶原酶裂解。Cthrc1 mRNA 表达水平因转化生长因子-β 和骨形态发生蛋白-4 的作用而增加。尽管 CTHRC1 与细胞外基质相关并包含一个短的胶原蛋白样基序，但与胶原蛋白家族其他成员不同，它在血管壁中的短暂表达与结构蛋白的功能相矛盾。一系列文章表明，CTHRC1 的过度表达通过血管生成、肿瘤相关巨噬细胞浸润和上皮间质转化等多种信号通路促进肿瘤进展^[1][2]。

Human

HEK293

C-6*His

Q96CG8-1 (S31-K243)

115908  [NCBI]

SEIPKGKQKAQLRQREVVDLYNGMCLQGPAGVPGRDGSPGANGIPGTPGIPGRDGFKGEKGECLRESFEESWTPNYKQCSWSSLNYGIDLGKIAECTFTKMRSNSALRVLFSGSLRLKCRNACCQRWYFTFNGAECSGPLPIEAIIYLDQGSPEMNSTINIHRTSSVEGLCEGIGAGLVDVAIWVGTCSDYPKGDASTGWNSVSRIIIEELPK

Approximately 24-32 kDa due to the glycosylation.

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Xusheng Ding, et al. CTHRC1 promotes gastric cancer metastasis via HIF-1α/CXCR4 signaling pathway. Biomed Pharmacother. 2020 Mar;123:109742.  [Content Brief]

  [2]. Peter Pyagay, et al. Collagen triple helix repeat containing 1, a novel secreted protein in injured and diseased arteries, inhibits collagen expression and promotes cell migration. Circ Res. 2005 Feb 4;96(2):261-8.  [Content Brief]