CTLA-4 蛋白, Human (Biotinylated, HEK293, mFc-Avi)

The CTLA-4 protein is a key inhibitory receptor and a major negative regulator of T cell responses in coordination of immune regulation. Its unique property is its significantly increased affinity for B7 ligands (CD80/CD86) compared with CD28. CTLA-4 Protein, Human (Biotinylated, HEK293, mFc-Avi) is the recombinant human-derived CTLA-4 protein, expressed by HEK293 , with C-Avi, C-mFc labeled tag. The total length of CTLA-4 Protein, Human (Biotinylated, HEK293, mFc-Avi) is 126 a.a., with molecular weight of 50-55 kDa.

GMP CTLA-4 是一种关键的抑制性受体，是在免疫调节的复杂框架内协调 T 细胞反应的主要负调节因子。这种调节功能源于 GMP CTLA-4 的独特特性，与同源刺激辅助受体 CD28 相比，其对其天然 B7 家族配体 CD80 和 CD86 的亲和力显着增强。这种结合亲和力的显着差异使 GMP CTLA-4 能够与 CD80/B7-1 和 CD86/B7.2 竞争性结合，对 T 细胞激活产生抑制影响并微调免疫反应。GMP CTLA-4 的同二聚体结构通过二硫键错综复杂地连接，进一步强调了其在免疫调节中作为分子哨兵的作用。此外，GMP CTLA-4 与 ICOSLG 相互作用，有助于其在免疫检查点通路中的多方面参与。

Measured by its binding ability in a functional ELISA. Immobilized Anti-CTLA4 antibody at 1 μg/mL can bind Biotinylated Human CTLA4. The EC ₅₀ is 10.57-15.25 ng/mL.

Human

HEK293

C-Avi;C-mFc

P16410 (A37-F162)

1493

50-55 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized a 0.22 μm filtered solution of PBS, 6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.