CXCL14/BRAK 蛋白, Mouse (His)

CXCL14/BRAK protein selectively attracts CESS B cells and THP-1 monocytes without affecting T cells. Its specific chemical attraction emphasizes its role in mediating B cell and monocyte migration, contributing to immune responses within the microenvironment. CXCL14/BRAK Protein, Mouse (His) is the recombinant mouse-derived CXCL14/BRAK protein, expressed by E. coli , with N-6*His labeled tag. The total length of CXCL14/BRAK Protein, Mouse (His) is 77 a.a., with molecular weight of ~13 kDa.

CXCL14/BRAK 蛋白作为趋化因子专门吸引 CESS B 细胞和 THP-1 单核细胞，但对 T 细胞没有趋化作用。这种选择性化学吸引强调了其在介导 B 细胞和单核细胞迁移中的作用，有助于微环境内的免疫反应。CXCL14/BRAK 独特的趋化特性突出了其对某些免疫细胞类型的特异性，表明其在调节 B 细胞和单核细胞的招募和激活方面具有特殊作用，同时不影响 T 细胞迁移。

The biological activity determined by a chemotaxis bioassay using THP-1 human monocytic leukemia cells.The ED₅₀ for this effect is 1.081 ng/mL, corresponding to a specific activity is 9.25×10⁵ units/mg.

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  The biological activity determined by a chemotaxis bioassay using THP-1 human monocytic leukemia cells.The ED₅₀ for this effect is 1.081 ng/mL, corresponding to a specific activity is 9.25×10⁵ units/mg.

Mouse

E. coli

N-6*His

Q9WUQ5 (S23-E99)

57266  [NCBI]

SKCKCSRKGPKIRYSDVKKLEMKPKYPHCEEKMVIVTTKSMSRYRGQEHCLHPKLQSTKRFIKWYNAWNEKRRVYEE

Approximately 13 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, 300 mM arginine, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.