CDK5 蛋白, Human (P.pastoris, His)

CDK5 is a proline-directed serine/threonine protein kinase that critically regulates neuronal cell cycle, differentiation and potential apoptosis in neuronal diseases by preventing cell cycle re-entry. It interacts with numerous proteins involved in neuronal development and coordinates processes such as survival, migration, differentiation, axonal growth, synaptogenesis, and neurotransmission. CDK5 Protein, Human (P.pastoris, His) is the recombinant human-derived CDK5 protein, expressed by P. pastoris , with N-His labeled tag. The total length of CDK5 Protein, Human (P.pastoris, His) is 292 a.a., with molecular weight of ~38.0 kDa.

CDK5 是一种脯氨酸导向的丝氨酸/苏氨酸蛋白激酶，在神经元细胞周期调节、分化以及通过启动细胞周期重新进入而可能诱导神经元疾病中的细胞凋亡中发挥重要作用。其相互作用涉及多种蛋白质，例如 D1 和 D3 型 G1 细胞周期蛋白、SRC、NOS3、VIM/波形蛋白、p35/CDK5R1、MEF2A、SIPA1L1、SH3GLB1、PXN、PAK1、MCAM/MUC18、SEPT5、SYN1、DNM1、 AMPH、SYNJ1、CDK16、RAC1、RHOA、CDC42、TONEBP/NFAT5、MAPT/TAU、MAP1B、组蛋白 H1、p53/TP53、HDAC1、APEX1、PTK2/FAK1、亨廷顿蛋白/HTT、ATM、MAP2、NEFH 和 NEFM。从功能上讲，CDK5 协调神经元发育中的关键过程，包括存活、迁移、分化、轴突和神经突生长、突触发生、少突胶质细胞分化、突触可塑性和神经传递，通过磷酸化关键蛋白来实现。此外，它还负向调节海马神经元胞体和突触末梢的 CACNA1B/CAV2.2 介导的 Ca(2+) 释放概率。在成熟的中枢神经系统中，CDK5 在神经递质运动和细胞存活中发挥着关键作用，激活抗凋亡蛋白 BCL2 和 STAT3，同时负向调节 JNK3/MAPK10 活性。其多方面功能涵盖各种细胞过程的复杂调节，包括 DNA 损伤反应、Wnt/β-连环蛋白信号传导和步态通路。此外，CDK5 影响树突棘形态发生，并通过调节 CLOCK 蛋白功能参与生物钟调节。

1.The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.
2.Measured in a cell proliferation assay using HuH-7 cells. The ED₅₀ for this effect is 2.516 ng/mL.

Human

P. pastoris

N-His

Q00535 (M1-P292)

1020  [NCBI]

MQKYEKLEKIGEGTYGTVFKAKNRETHEIVALKRVRLDDDDEGVPSSALREICLLKELKHKNIVRLHDVLHSDKKLTLVFEFCDQDLKKYFDSCNGDLDPEIVKSFLFQLLKGLGFCHSRNVLHRDLKPQNLLINRNGELKLADFGLARAFGIPVRCYSAEVVTLWYRPPDVLFGAKLYSTSIDMWSAGCIFAELANAGRPLFPGNDVDDQLKRIFRLLGTPTEEQWPSMTKLPDYKPYPMYPATTSLVNVVPKLNATGRDLLQNLLKCNPVQRISAEEALQHPYFSDFCPP

Approximately 38.0 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4.

<1.0 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.