1. 重组蛋白
  2. CD Antigens Fc Receptors
  3. T Cell CD Proteins NK Cell CD Proteins Macrophage CD Proteins Dendritic Cell CD Proteins Fc-gamma Receptor
  4. FcγRIIIA/CD16a Fc gamma RIII/CD16
  5. Fc gamma RIIIA/CD16a 蛋白, Cynomolgus (Biotinylated, HEK293, His-Avi)

Fc gamma RIIIA/CD16a 蛋白, Cynomolgus (Biotinylated, HEK293, His-Avi)

目录号: HY-P78879
COA 产品使用指南 技术支持

FcγRIIIA/CD16a 蛋白是 IgG 的受体,在与聚集的抗原-IgG 复合物结合时被最佳激活,从而诱导抗体依赖性细胞毒性 (ADCC)。它介导自然杀伤细胞的 IgG 效应功能,限制病毒载量。Fc gamma RIIIA/CD16a 蛋白, Cynomolgus (Biotinylated, HEK293, His-Avi) 是重组的 Fc gamma RIIIA/CD16a 蛋白,由 HEK293 表达,带有 C-Avi, C-His 标签。Fc gamma RIIIA/CD16a 蛋白, Cynomolgus (Biotinylated, HEK293, His-Avi) 全长 192 个氨基酸,分子量为 36-45 kDa。

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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

The FcγRIIIA/CD16a protein is a receptor for IgG and is optimally activated upon binding of aggregated antigen-IgG complexes, inducing antibody-dependent cellular cytotoxicity (ADCC). It mediates IgG effector function on natural killer cells, limiting viral load. Fc gamma RIIIA/CD16a Protein, Cynomolgus (Biotinylated, HEK293, His-Avi) is the recombinant cynomolgus-derived Fc gamma RIIIA/CD16a protein, expressed by HEK293 , with C-Avi, C-His labeled tag. The total length of Fc gamma RIIIA/CD16a Protein, Cynomolgus (Biotinylated, HEK293, His-Avi) is 192 a.a., with molecular weight of 36-45 kDa.

研究背景

FcγRIIIA/CD16a 蛋白作为免疫球蛋白 γ (IgG) 恒定 Fc 片段的受体,在与细胞表面展示的聚集抗原-IgG 复合物结合时最佳激活,从而产生抗体依赖性细胞毒性 (ADCC)。它介导自然杀伤 (NK) 细胞上的 IgG 效应器功能,触发细胞因子的产生、脱颗粒并限制感染期间的病毒载量。此外,FcγRIIIA 参与记忆样适应性 NK 细胞的生成,可通过 ADCC 有效消除病毒感染的细胞并调节 NK 细胞的存活和增殖。作为 Fc 结合亚基,它与 CD247 和/或 FCER1G 接头结合形成功能性信号传导复合物,启动细胞内信号传导途径,例如磷脂酰肌醇 3-激酶信号传导和钙流,这对 NK 细胞激活至关重要。FcγRIIIA 还共刺激 NK 细胞,独立于 IgG 结合触发靶细胞裂解。它在介导治疗性抗体的抗肿瘤活性中发挥作用,并且在连接到单核细胞上后,诱导 IgG 包被的肿瘤细胞发生肿瘤坏死因子-α (TNFA) 依赖性 ADCC。此外,FcγRIIIA 与 CD2 相互作用,有助于 NK 细胞活化和细胞毒性,并与 S100A4 相互作用,抑制 FCGR3A 的 PKC 依赖性磷酸化。

生物活性

Measured by its binding ability in a functional ELISA. Immobilized Anti-CD16a antibody at 2 μg/mL can bind Biotinylated Cynomolgus CD16. The EC50 is 1.737-10.78 ng/mL.

种属

Cynomolgus

表达系统

HEK293

标签

C-Avi;C-His

蛋白编号

Q8SPW2 (G17-Q208)

基因 ID
同用名
Low affinity immunoglobulin gamma Fc region receptor III-A; FcRIIIa; FcR-10; CD16a; FCGR3A; IGFR3
分子量

36-45 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末

组分

Lyophilized a 0.22 μm filtered solution of 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Fc gamma RIIIA/CD16a Protein, Cynomolgus (Biotinylated, HEK293, His-Avi)
目录号:
HY-P78879
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