Cystatin B/CSTB 蛋白, Human (His)

Cystatin B (CSTB) Protein is a member of the cystatin family. Cystatin B/CSTB Protein, Human (His) is the recombinant human-derived Cystatin B/CSTB protein, expressed by E. coli , with N-His labeled tag. The total length of Cystatin B/CSTB Protein, Human (His) is 97 a.a., with molecular weight of ~15 kDa.

Cystatin B/CSTB 蛋白作为细胞内硫醇蛋白酶抑制剂发挥作用。胱抑素 B/CSTB 蛋白能够形成通过非共价力稳定的二聚体，抑制木瓜蛋白酶和组织蛋白酶 l、h 和 b。胱抑素 B/CSTB 蛋白被认为在防止蛋白酶从溶酶体泄漏方面发挥作用。有证据表明，胱抑素 B/CSTB 基因突变是造成进行性肌阵挛癫痫 (EPM1) 患者主要缺陷的原因。半胱氨酸蛋白酶抑制剂超家族包括含有多个半胱氨酸蛋白酶抑制剂样序列的蛋白质。超家族中有3个抑制家族，包括1型半胱氨酸蛋白酶抑制剂（stefins）、2型半胱氨酸蛋白酶抑制剂和激肽原。

Measured by its ability to inhibit papain cleavage of a fluorogenic peptide substrate Z-FR-AMC. The IC₅₀ value is < 30 nM.

Human

E. coli

N-His

Q76LA1 (M2-F98)

1476  [NCBI]

MCGAPSATQPATAETQHIADQVRSQLEEKENKKFPVFKAVSFKSQVVAGTNYFIKVHVGDEDFVHLRVFQSLPHENKPLTLSNYQTNKAKHDELTYF

Approximately 15 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris, 50 mM NaCl, pH 8.0 or 50 mM Tris-HCL, 300 mM NaCl, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.