Cystatin C/CST3 蛋白, Human (HEK293, His)

Cystatin C/CST3 protein can significantly inhibit cysteine proteases and locally regulate their enzymatic activity. It captures and binds free plasma hemoglobin, preventing kidney damage and supporting hepatic heme iron recycling. Cystatin C/CST3 Protein, Human (HEK293, His) is the recombinant human-derived Cystatin C/CST3 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Cystatin C/CST3 Protein, Human (HEK293, His) is 120 a.a., with molecular weight of 15-18 kDa.

胱抑素 C/CST3 蛋白作为半胱氨酸蛋白酶的抑制剂发挥着至关重要的生理作用，充当其酶活性的局部调节剂。众所周知，它可以捕获并结合游离血浆血红蛋白，防止肾脏损伤并实现血红素铁的肝脏循环利用。在溶血的情况下，血红蛋白可以在肾脏中积聚并分泌到尿液中，胱抑素 C/CST3 在清除血红蛋白和触珠蛋白之间形成的复合物方面发挥着至关重要的作用。此外，胱抑素 C/CST3 还可作为抗氧化剂，表现出抗菌活性，并有助于调节急性期反应的各个方面。该蛋白质的同二聚体结构进一步增强了其作为酶抑制剂的功能和有效性。

Measured by its ability to inhibit papain cleavage of a fluorogenic peptide substrate Z-FR-AMC. The IC₅₀ value is 91.68 nM.

Human

HEK293

C-His

P01034 (S27-A146)

1471  [NCBI]

SSPGKPPRLVGGPMDASVEEEGVRRALDFAVGEYNKASNDMYHSRALQVVRARKQIVAGVNYFLDVELGRTTCTKTQPNLDNCPFHDQPHLKRKAFCSFQIYAVPWQGTMTLSKSTCQDA

15-18 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HCl, 500 mM NaCl, 0.01% Tween 80, 1 mM EDTA, 50% Glycerol, pH 8.0 or PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.