2. 重组蛋白
  3. Enzymes & Regulators
  4. Isomerases (EC 5)
  5. DCT 蛋白, Human (His-SUMO)

DCT 蛋白, Human (His-SUMO)

目录号: HY-P72168
产品使用指南

DCT 蛋白在黑色素生物合成中至关重要，可催化 L-多巴色素转化为 5,6-二羟基吲哚-2-羧酸 (DHICA)，这是黑色素合成的关键步骤。DCT 蛋白, Human (His-SUMO) 是重组的 DCT 蛋白，由 E. coli 表达，带有 N-SUMO, N-10*His 标签。DCT 蛋白, Human (His-SUMO) 全长 449 个氨基酸，分子量约为 ~71.3 kDa。

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描述

DCT Protein is essential in melanin biosynthesis, catalyzing the conversion of L-dopachrome to 5,6-dihydroxyindole-2-carboxylic acid (DHICA), a pivotal step in melanin synthesis. DCT Protein, Human (His-SUMO) is the recombinant human-derived DCT protein, expressed by E. coli , with N-SUMO, N-10*His labeled tag. The total length of DCT Protein, Human (His-SUMO) is 449 a.a., with molecular weight of ~71.3 kDa.
研究背景

DCT蛋白在黑色素的生物合成中起着至关重要的作用。它负责催化L-多巴色素转化为5,6-二羟基吲哚-2-羧酸（DHICA），这是黑色素合成的关键步骤。
种属

Human
表达系统

E. coli
标签

N-SUMO;N-10*His
蛋白编号

P40126 (Q24-T472)
基因 ID
蛋白结构
N-term
10*His-SUMO
DCT (Q24-T472)
Accession # P40126
C-term
同用名
L dopachrome tautomerase; DCT; Dopachrome delta isomerase; Dopachrome tautomerase; Dopachrome tautomerase dopachrome delta isomerase; tyrosine related protein 2; DT; EC 5.3.3.12; L dopachrome Delta isomerase; L-dopachrome Delta-isomerase; L-dopachrome tautomerase; TRP 2; TRP-2; TRP2; Tyrosinase related protein 2; Tyrosinase-related protein 2; TYRP2; TYRP2_HUMAN
氨基酸序列

QFPRVCMTVDSLVNKECCPRLGAESANVCGSQQGRGQCTEVRADTRPWSGPYILRNQDDRELWPRKFFHRTCKCTGNFAGYNCGDCKFGWTGPNCERKKPPVIRQNIHSLSPQEREQFLGALDLAKKRVHPDYVITTQHWLGLLGPNGTQPQFANCSVYDFFVWLHYYSVRDTLLGPGRPYRAIDFSHQGPAFVTWHRYHLLCLERDLQRLIGNESFALPYWNFATGRNECDVCTDQLFGAARPDDPTLISRNSRFSSWETVCDSLDDYNHLVTLCNGTYEGLLRRNQMGRNSMKLPTLKDIRDCLSLQKFDNPPFFQNSTFSFRNALEGFDKADGTLDSQVMSLHNLVHSFLNGTNALPHSAANDPIFVVLHSFTDAIFDEWMKRFNPPADAWPQELAPIGHNRMYNMVPFFPPVTNEELFLTSDQLGYSYAIDLPVSVEETPGWPTT

分子量

Approximately 71.3 kDa
纯度

Greater than 90% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

DCT Protein, Human (His-SUMO) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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