Delta-like 蛋白 1/DLL1 蛋白, Rat (HEK293, His)

Delta-like protein 1 (DLL1) is a transmembrane ligand for NOTCH1, NOTCH2, and NOTCH3 receptors and participates in cis- and trans-interactions with their extracellular domains. Upon interaction, DLL1 induces mechanical forces that promote Notch signaling by triggering NICD nuclear accumulation. Delta-like protein 1/DLL1 Protein, Rat (HEK293, His) is the recombinant rat-derived Delta-like protein 1/DLL1 protein, expressed by HEK293 , with C-His labeled tag. The total length of Delta-like protein 1/DLL1 Protein, Rat (HEK293, His) is 517 a.a., with molecular weight of ~81.7 kDa.

Delta 样蛋白 1 (DLL1) 是 NOTCH1、NOTCH2 和 NOTCH3 受体的跨膜配体蛋白，与 Notch 受体的胞外结构域 (ECD) 进行顺式和反式相互作用。反式相互作用后，配体细胞通过网格蛋白介导的内吞作用、配体泛素化、EPN1 相互作用和肌动蛋白聚合诱导机械力。这些事件促进Notch受体胞外域(NECD)转内吞作用，通过Notch受体(NICD)胞内域的裂解、过度磷酸化和核积累来触发Notch信号传导。DLL1 对于胚胎发育以及各种组织和免疫系统中成体干细胞的维持至关重要，通过分化和增殖效应调节细胞谱系、规格、模式和形态发生。它在大脑发育、调节不同环境下的神经元分化和神经发生中发挥着至关重要的作用。DLL1 还有助于免疫系统的发育，抑制 B 细胞谱系分化，同时促进 T 细胞/NK 细胞前体的出现。此外，DLL1 调节肌肉发育、胰腺细胞发育、动脉特性维持、血管生成、肠道杯状细胞分化、内耳发育、肾单位发育以及各种生理过程，包括生长、血压和能量稳态。DLL1 与多种蛋白质相互作用，包括 TJP1、MAGI1、PSEN1、MFAP5、MIB1、NEURL1B、SYNJ2BP、MAGI2、MAGI3、MPDZ 和 NOTCH1，在多种细胞过程中发挥多方面的作用。

Measured by the ability of the immobilized protein to enhance BMP-2 induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. The ED₅₀ for this effect is approximately 1.431 μg/mL in the presence of 500 ng/mL of BMP-2, corresponding to a specific activity is 698.812 units/mg.

Rat

HEK293

C-10*His

P97677 (Q18-S534)

84010  [NCBI]

QVWSSGVFELKLQEFVNKKGLLGNRNCCRGGSGPPCACRTFFRVCLKHYQASVSPEPPCTYGSAVTPVLGVDSFSLPDGAGIDPAFSNPIRFPFGFTWPGTFSLIIEALHTDSPDDLATENPERLISRLTTQRHLTVGEEWSQDLHSSGRTDLRYSYRFVCDEHYYGEGCSVFCRPRDDAFGHFTCGERGEKMCDPGWKGQYCTDPICLPGCDDQHGYCDKPGECKCRVGWQGRYCDECIRYPGCLHGTCQQPWQCNCQEGWGGLFCNQDLNYCTHHKPCRNGATCTNTGQGSYTCSCRPGYTGANCELEVDECAPSPCRNGGSCTDLEDSYSCTCPPGFYGKVCELSAMTCADGPCFNGGRCSDNPDGGYTCHCPAGFSGFNCEKKIDLCSSSPCSNGAKCVDLGNSYLCRCQTGFSGRYCEDNVDDCASSPCANGGTCRDSVNDFSCTCPPGYTGRNCSAPVSRCEHAPCHNGATCHQRGQRYMCECAQGYGGANCQFLLPEPPPDLIVAAQGGS

Approximately 55-60 kDa due to the glycosylation.

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.