DENND1A 蛋白, Human (His-SUMO)

The DENND1A protein is a guanine nucleotide exchange factor (GEF) that coordinates clathrin-mediated endocytosis through activation of RAB35. It catalyzes the conversion of inactive GDP-bound RAB35 into its active GTP-bound state, regulating synaptic vesicle dynamics and other processes. DENND1A Protein, Human (His-SUMO) is the recombinant human-derived DENND1A, expressed by E. coli , with N-SUMO, N-6*His labeled tag. The total length of DENND1A Protein, Human (His-SUMO) is 559 a.a.,

DENND1A 是一种鸟嘌呤核苷酸交换因子 (GEF)，通过激活 RAB35 在协调网格蛋白介导的内吞作用中发挥着关键作用。其功能涉及催化 GDP 到 GTP 的交换，从而将 RAB35 的非活性 GDP 结合形式转化为其活性 GTP 结合状态。值得注意的是，DENND1A 是网格蛋白介导的内吞过程的关键调节因子，影响突触小泡动力学并促进早期内体的退出。此外，DENND1A 对磷脂酰肌醇磷酸 (PtdInsPs) 表现出结合亲和力，并优先结合 PtdIns(3)P。有趣的是，其 GEF 活性受到自抑制，可能是由 DENN 结构域和 C 末端之间的分子内相互作用介导的，而激活是通过破坏这种自抑制机制来通过 Akt 激活来调节的。

Human

E. coli

N-SUMO;N-6*His

Q8TEH3-2 (M1-H559)

57706  [NCBI]

MGSRIKQNPETTFEVYVEVAYPRTGGTLSDPEVQRQFPEDYSDQEVLQTLTKFCFPFYVDSLTVSQVGQNFTFVLTDIDSKQRFGFCRLSSGAKSCFCILSYLPWFEVFYKLLNILADYTTKRQENQWNELLETLHKLPIPDPGVSVHLSVHSYFTVPDTRELPSIPENRNLTEYFVAVDVNNMLHLYASMLYERRILIICSKLSTLTACIHGSAAMLYPMYWQHVYIPVLPPHLLDYCCAPMPYLIGIHLSLMEKVRNMALDDVVILNVDTNTLETPFDDLQSLPNDVISSLKNRLKKVSTTTGDGVARAFLKAQAAFFGSYRNALKIEPEEPITFCEEAFVSHYRSGAMRQFLQNATQLQLFKQFIDGRLDLLNSGEGFSDVFEEEINMGEYAGSDKLYHQWLSTVRKGSGAILNTVKTKANPAMKTVYKFAKDHAKMGIKEVKNRLKQKDIAENGCAPTPEEQLPKTAPSPLVEAKDPKLREDRRPITVHFGQVRPPRPHVVKRPKSNIAVEGRRTSVPSPEQNTIATPATLHILQKSITHFAAKFPTRGWTSSSH

Approximately 79.4 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.