DHH 蛋白, Human (C23II)

DHH Protein, Human is a single non-glycosylated polypeptide chain. Desert hedgehog protein (DHH) is a member of the Hedgehog family which encodes signaling molecules that play an important role in regulating morphogenesis.

沙漠刺猬 (DHH) 属于刺猬基因家族，充当分泌性细胞间信号转导器。DHH 是小鼠和人类正常睾丸发育和功能所必需的形态发生素，但在鸟类谱系中不存在。与其他刺猬蛋白一样，DHH 通过补丁 (PTCH) 受体 1 和 2 发出信号。受体 PTCH1 和 PTCH2 是发育中和成年有袋动物性腺中高度保守的刺猬信号传导介质。这些发现共同表明 DHH 是性腺发育和配子发生过程中必需的兽类哺乳动物特异性形态发生素^[1]。

Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. The ED50 this effect is 6.275 μg/mL, corresponding to a specific activity is 159.363 units/mg.

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  Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. The ED₅₀ this effect is 6.275 μg/mL, corresponding to a specific activity is 159.363 units/mg.

Human

E. coli

Tag Free

O43323 (G24-G198, C23II)

50846  [NCBI]

IIGPGRGPVGRRRYARKQLVPLLYKQFVPGVPERTLGASGPAEGRVARGSERFRDLVPNYNPDIIFKDEENSGADRLMTERCKERVNALAIAVMNMWPGVRLRVTEGWDEDGHHAQDSLHYEGRALDITTSDRDRNKYGLLARLAVEAGFDWVYYESRNHVHVSVKADNSLAVRAGG

Approximately 20.85 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. O'Hara WA, et al. Desert hedgehog is a mammal-specific gene expressed during testicular and ovarian development in a marsupial. BMC Dev Biol. 2011 Dec 1;11:72.  [Content Brief]