DMBT1 蛋白, Human (HEK293, His)

The DMBT1 protein is a potential tumor suppressor in a variety of cancers and affects a variety of biological processes. It contributes to mucosal and cellular immune defense, epithelial differentiation, and serves as an opsonin receptor for SFTPD and SPAR in macrophages. DMBT1 Protein, Human (HEK293, His) is the recombinant human-derived DMBT1 protein, expressed by HEK293 , with C-His labeled tag. The total length of DMBT1 Protein, Human (HEK293, His) is 201 a.a., with molecular weight of 35-45 kDa.

DMBT1 蛋白作为一种多方面的候选者出现，在脑癌、肺癌、食道癌、胃癌和结直肠癌中具有潜在的肿瘤抑制作用。除了对癌症的影响之外，DMBT1 还参与多种生物过程，在粘膜防御系统、细胞免疫防御和上皮分化中发挥作用。它在全身巨噬细胞组织（包括胃肠道上皮细胞）中充当 SFTPD 和 SPAR 的调理素受体。此外，DMBT1 在肝再生中发挥作用，并且是肝谱系内转运放大导管（卵圆）细胞命运决定和分化的关键因素。值得注意的是，它在早期胚胎发生过程中参与柱状上皮细胞的终末分化。此外，DMBT1 在唾液中充当结合蛋白，可能调节味觉。它与 HIV-1 包膜蛋白结合，在抑制和促进病毒传播方面发挥双重作用。该蛋白质对革兰氏阳性和革兰氏阴性细菌表现出广泛的钙依赖性结合谱，表明在防御细菌病原体方面具有重要作用。DMBT1 的多种相互作用，包括与肌动蛋白细胞骨架的关联以及在受调节的胞吐作用期间参与其重塑，突出了其在细胞过程中的多功能功能。其与胰腺酶原的 pH 依赖性相互作用表明其在胰腺腺泡细胞的调节分泌途径中作为高尔基体货物受体的潜在作用，进一步强调了 DMBT1 在各种生理环境中的复杂性和重要性。

Measured by its binding ability in a functional ELISA. Immobilized recombinant human Galectin-3 at 10 μg/mL (100 μl/well) can bind biotinylated DMBT1-His with a linear range of 0.06-1.0 μg/mL.

Human

HEK293

C-His

Q9UGM3/NP_004397.2 (T20-S220)

1755  [NCBI]

TGGWIPRTTDYASLIPSEVPLDPTVAEGSPFPSESTLESTVAEGSPISLESTLESTVAEGSLIPSESTLESTVAEGSDSGLALRLVNGDGRCQGRVEILYRGSWGTVCDDSWDTNDANVVCRQLGCGWAMSAPGNAWFGQGSGPIALDDVRCSGHESYLWSCPHNGWLSHNCGHGEDAGVICSAAQPQSTLRPESWPVRIS

35-45 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4, 5% Trehalose, 5% Mannitol, 0.01% Tween-80 or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.