DNAM-1 蛋白, Human (Biotinylated, HEK293, His-Avi)

DNAM-1 protein is a key player in cell-cell adhesion and lymphocyte signaling, mediating cytotoxicity and lymphokine secretion of CTL and NK cells. As a receptor for NECTIN2, DNAM-1 stimulates T cell proliferation and cytokine production (IL2, IL5, IL10, IL13, and IFNG) upon ligand binding. DNAM-1 Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived DNAM-1 protein, expressed by HEK293 , with C-Avi, C-His labeled tag. The total length of DNAM-1 Protein, Human (Biotinylated, HEK293, His-Avi) is 229 a.a., with molecular weight of 48-68 kDa.

DNAM-1 蛋白与细胞毒性 T 淋巴细胞 (CTL) 和自然杀伤 (NK) 细胞介导的细胞间粘附、淋巴细胞信号传导、细胞毒性和淋巴因子分泌密切相关。DNAM-1 作为 NECTIN2 的细胞表面受体，在与配体结合后，刺激 T 细胞增殖和细胞因子（如 IL2、IL5、IL10、IL13 和 IFNG）的产生。此外，DNAM-1 与 PVRIG 竞争 NECTIN2 结合，强调了其在细胞相互作用和信号通路中的调节作用。该蛋白还与 PVR 和 NECTIN2 相互作用，有助于其在免疫反应和细胞通讯中的多方面功能。

Measured by its binding ability in SPR assay. Human CD155 hFc immobilized on CM5 Chip can bind Biotinylated Human DNAM-1 His-Avi with an affinity constant of 0.503 μM.

Human

HEK293

C-Avi;C-His

Q15762 (E19-N247)

10666  [NCBI]

48-68 kDa

Greater than 95% as determined by Tris-Bis PAGE.

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 5% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.