DNAM-1 蛋白, Rat (HEK293, His)

DNAM-1 protein is a key player in cell-cell adhesion and lymphocyte signaling, mediating cytotoxicity and lymphokine secretion of CTL and NK cells. As a receptor for NECTIN2, DNAM-1 stimulates T cell proliferation and cytokine production (IL2, IL5, IL10, IL13, and IFNG) upon ligand binding. DNAM-1 Protein, Rat (HEK293, His) is the recombinant rat-derived DNAM-1 protein, expressed by HEK293 , with C-His labeled tag. The total length of DNAM-1 Protein, Rat (HEK293, His) is 234 a.a., with molecular weight of ~28 KDa.

DNAM-1 蛋白与细胞毒性 T 淋巴细胞 (CTL) 和自然杀伤 (NK) 细胞介导的细胞间粘附、淋巴细胞信号传导、细胞毒性和淋巴因子分泌密切相关。DNAM-1 作为 NECTIN2 的细胞表面受体，在与配体结合后，刺激 T 细胞增殖和细胞因子（如 IL2、IL5、IL10、IL13 和 IFNG）的产生。此外，DNAM-1 与 PVRIG 竞争 NECTIN2 结合，强调了其在细胞相互作用和信号通路中的调节作用。该蛋白还与 PVR 和 NECTIN2 相互作用，有助于其在免疫反应和细胞通讯中的多方面功能。

Measured by its binding ability in a functional ELISA. Immobilized Rat DNAM-1 at 1 μg/mL (100 μL/well) can bind biotinylated CD155 Protein. The ED₅₀ for this effect is 7.119 ng/mL.

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  Measured by its binding ability in a functional ELISA. Immobilized Rat DNAM-1 at 1μg/mL (100 μL/well) can bind biotinylated CD155 Protein. The ED₅₀ for this effect is 7.119 ng/mL.

Rat

HEK293

C-His

D3ZS97 (E32-I265)

307199  [NCBI]

EETLWDTTVRLSETMTLECVYPLTDNLTQAEWTKDTGTKKVNIAVYHPNHNMHIDPNYLHRVHFQNATVGFRNMSLSFYNASEADIGIYTCLFHAFPRGSWEKKIKVVQSDSFETTAPLDSYLSAEPGQNVTLSCQLERTWLVQLVIWEKVQPHQVDILASCNLSQGTRYTSKYLRQTWSDCSQESMKSALIIPYAMAADSGLYRCRSEASTGTNKTCVIRLIITDGGTNNHFI

Approximately 28 kDa.

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.