DUSP14 蛋白, Human (His)

DUSP14 protein can inactivate MAP kinase and dephosphorylate ERK, JNK and p38 MAP kinase. Its negative regulation extends to TCR signaling, where DUSP14 dephosphorylates the MAP3K7 adapter TAB1, resulting in inactivation. DUSP14 Protein, Human (His-MBP) is the recombinant human-derived DUSP14 protein, expressed by E. coli , with N-His labeled tag.

DUSP14 蛋白在 MAP 激酶失活中发挥着至关重要的作用，对 ERK、JNK 和 p38 MAP 激酶表现出去磷酸化活性。其负调节功能延伸至 TCR 信号传导，其中 DUSP14 使 MAP3K7 接头 TAB1 去磷酸化，导致其失活。这种机制强调了 DUSP14 在调节 TCR 信号通路中的作用，揭示了它参与调节免疫反应。DUSP14 对 MAP 激酶级联关键成分的去磷酸化活性凸显了其微调细胞信号传导的能力，对受 MAP 激酶途径影响的各种生理过程具有潜在影响。

Measured in a cell proliferation assay using A549 cells. The ED₅₀ for this effect is ≤8.219 ng/mL, corresponding to a specific activity is ≥1.217×10⁵ units/mg.

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  Measured in a cell proliferation assay using A549 cells. The ED₅₀ for this effect is 5.031 ng/mL, corresponding to a specific activity is 1.988×10⁵ units/mg.

Human

E. coli

N-6*His

O95147 (M1-H191)

11072  [NCBI]

MSSRGHSTLPRTLMAPRMISEGDIGGIAQITSSLFLGRGSVASNRHLLQARGITCIVNATIEIPNFNWPQFEYVKVPLADMPHAPIGLYFDTVADKIHSVSRKHGATLVHCAAGVSRSATLCIAYLMKFHNVCLLEAYNWVKARRPVIRPNVGFWRQLIDYERQLFGKSTVKMVQTPYGIVPDVYEKESRH

Approximately 21-23 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4, 5% trehalose, 5% mannitol and 0.01% Tween80 or PBS, pH 7.4, 8% trehalose, 8% mannitol and 0.02% Tween80.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.