1. 重组蛋白
  2. Viral Proteins
  3. Ebola Virus Proteins
  4. Ebola Virus GP Proteins
  5. Ebola virus Glycoprotein/GP 蛋白 (AHX24649, sf9, His)

Ebola virus Glycoprotein/GP 蛋白 (AHX24649, sf9, His)

目录号: HY-P75273
产品使用指南

埃博拉病毒糖蛋白(GP)在病毒进入过程中发挥着关键作用,其中GP1作为受体结合亚基,GP2用于膜融合。GP 下调宿主细胞表面分子,破坏粘附,并可能导致血管完整性破坏。Ebola virus Glycoprotein/GP 蛋白 (AHX24649, sf9, His) 是重组的 埃博拉病毒糖蛋白/GP 蛋白,由 Sf9 insect cells 表达,带有 C-His 标签。

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描述

Ebola virus glycoproteins (GPs) play a key role in the virus entry process, with GP1 serving as the receptor-binding subunit and GP2 for membrane fusion.GP downregulates host cell surface molecules, disrupts adhesion and may lead to disruption of vascular integrity.Ebola virus Glycoprotein/GP Protein (AHX24649, sf9, His) is the recombinant Virus-derived Ebola virus Glycoprotein/GP protein, expressed by Sf9 insect cells , with C-His labeled tag.

研究背景

埃博拉病毒糖蛋白 (GP) 的三聚体 GP1,2 复合物在病毒进入过程中发挥着至关重要的作用,其中 GP1 充当受体结合亚基,GP2 充当膜融合亚基。在感染后期,GP下调各种宿主细胞表面分子的表达,包括整合素,如ITGA1、ITGA2、ITGA3、ITGA4、ITGA5、ITGA6、ITGAV和ITGB1,破坏细胞粘附并导致细胞脱离,可能导致血管完整性破坏和出血。GP 还与宿主 TLR4 相互作用,刺激单核细胞的分化和激活,导致 T 淋巴细胞的旁观者死亡。此外,GP可以抵消宿主BST2/tetherin的抗病毒作用,与VP40和宿主BST2合作激活经典的NF-kappa-B通路,并作为抗GP1,2抗体的诱饵,有助于病毒免疫逃避。此外,GP 与宿主巨噬细胞和树突状细胞相互作用并激活它们,通过激活宿主 TLR4 诱导细胞因子转录上调。

种属

Virus

表达系统

Sf9 insect cells

标签

C-His

蛋白编号

AHX24649 (I33-Q650)

基因 ID

/

蛋白结构
N-term
EBOV GP (I33-Q650)
Accession # AHX24649
His
C-term
同用名
Ebola virus EBOV (subtype Zaire, strain H.sapiens-wt/GIN/2014/Kissidougou-C15) Glycoprotein / GP Protein (His)
分子量

Approximately 69.3 kDa

纯度

Greater than 85% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

Ebola virus Glycoprotein/GP Protein (AHX24649, sf9, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Ebola virus Glycoprotein/GP Protein (AHX24649, sf9, His)
目录号:
HY-P75273
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