Ebola virus VP40/Matrix VP40 蛋白 (Q5XX06, His)

Ebola virus VP40 (matrix VP40 protein) centrally directs virus assembly and budding and has complex interactions with viral ribonucleocapsid and host ESCRT proteins (VPS4, PDCD6IP/ALIX, NEDD4, TGS101). Ebola virus VP40/Matrix VP40 Protein (Q5XX06, His) is the recombinant Virus-derived Ebola virus VP40/Matrix VP40 protein, expressed by E. coli , with N-His labeled tag. The total length of Ebola virus VP40/Matrix VP40 Protein (Q5XX06, His) is 296 a.a., with molecular weight of ~34.5 kDa.

埃博拉病毒 VP40，也称为基质 VP40 蛋白，在病毒颗粒组装和出芽、协调与病毒和宿主成分之间复杂的相互作用中发挥着核心作用。它通过与病毒核糖核衣壳和宿主 ESCRT 系统成员（包括 VPS4、PDCD6IP/ALIX、NEDD4 或 TGS101）相互作用来发挥作用，这对于有效出芽至关重要。此外，它与宿主 E3 泛素连接酶 SMURF2 的结合促进病毒出芽。值得注意的是，VP40 可能通过包装到外泌体中，通过 RNAi 抑制和外泌体旁观者凋亡降低受体细胞的活力，从而导致免疫细胞功能障碍。VP40 以各种寡聚形式存在，例如同二聚体、对出芽至关重要的同聚六聚体以及参与基因组复制和 RNA 结合的同八聚体，VP40 在质膜上使用磷脂酰肌醇 4,5-二磷酸 (PI(PI( 4,5)P2)。这些六聚体对于出芽过程至关重要，而八聚体则在基因组复制和 RNA 结合中发挥作用。VP40 进一步与包括 TSG101、NEDD4、PDCD6IP/ALIX、SMURF2、ITCH 和核蛋白/NP 在内的宿主因子相互作用，突出了其在控制病毒组装和流出方面的关键作用。

Virus

E. coli

N-His

Q5XX06 (V31-K326)

3160775  [NCBI]

VSGIQQKQEVLPGMDTPSNSMRPVADDNIDHTSHTPNGVASAFILEATVNVISGPKVLMKQIPIWLPLGIADQKTYSFDSTTAAIMLASYTITHFGKANNPLVRVNRLGQGIPDHPLRLLRMGNQAFLQEFVLPPVQLPQYFTFDLTALKLVTQPLPAATWTDETPSNLSGALRPGLSFHPKLRPVLLPGKTGKKGHVSDLTAPDKIQTIVNLMQDFKIVPIDPAKSIIGIEVPELLVHKLTGKKMSQKNGQPIIPVLLPKYIGLDPISPGDLTMVITPDYDDCHSPASCSYLSEK

Approximately 34.5 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.