1. 重组蛋白
  2. Enzymes & Regulators
  3. Hydrolases (EC 3)
  4. ECE-1 蛋白, Human (HEK293, His)

ECE-1 蛋白, Human (HEK293, His)

目录号: HY-P70035
产品使用指南

ECE-1 蛋白通过催化大内皮素-1 转化为内皮素-1,在血管调节中发挥关键作用。这一酶促过程是内皮素-1 成熟的关键步骤,内皮素-1 是一种有效的血管收缩剂和信号分子,参与血压、血管张力和各种生理功能的调节。ECE-1 蛋白, Human (HEK293, His) 是重组的 ECE-1 蛋白,由 HEK293 表达,带有 N-8*His 标签。ECE-1 蛋白, Human (HEK293, His) 全长 681 个氨基酸,分子量为 94-130 kDa。

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描述

The ECE-1 protein plays a key role in vascular regulation by catalyzing the conversion of large endothelin-1 to endothelin-1. This enzymatic process is a critical step in the maturation of endothelin-1, a potent vasoconstrictor and signaling molecule involved in the regulation of blood pressure, vascular tone, and various physiological functions. ECE-1 Protein, Human (HEK293, His) is the recombinant human-derived ECE-1 protein, expressed by HEK293 , with N-8*His labeled tag. The total length of ECE-1 Protein, Human (HEK293, His) is 681 a.a., with molecular weight of 94-130 kDa.

研究背景

ECE-1 蛋白是参与大内皮素-1 向内皮素-1 转化的关键酶。这种转化对于内皮素-1 的成熟和激活起着关键作用,内皮素-1 是一种具有有效血管收缩作用的肽。通过催化大内皮素-1 的裂解,ECE-1 有助于调节血管张力和血压。内皮素-1 的激活对多种生理过程有影响,包括心血管稳态和平滑肌收缩。作为内皮素系统中的关键酶,ECE-1 的酶活性对于调节血管功能和维持心血管健康至关重要(参考:未提供)。

种属

Human

表达系统

HEK293

标签

N-8*His

蛋白编号

P42892 (Q90-W770)

基因 ID
蛋白结构
N-term
8*His
ECE-1 (Q90-W770)
Accession # P42892
C-term
同用名
rHuEndothelin-converting enzyme 1/ECE-1, His; Endothelin-converting enzyme 1; ECE-1
氨基酸序列

QYQTRSPSVCLSEACVSVTSSILSSMDPTVDPCHDFFSYACGGWIKANPVPDGHSRWGTFSNLWEHNQAIIKHLLENSTASVSEAERKAQVYYRACMNETRIEELRAKPLMELIERLGGWNITGPWAKDNFQDTLQVVTAHYRTSPFFSVYVSADSKNSNSNVIQVDQSGLGLPSRDYYLNKTENEKVLTGYLNYMVQLGKLLGGGDEEAIRPQMQQILDFETALANITIPQEKRRDEELIYHKVTAAELQTLAPAINWLPFLNTIFYPVEINESEPIVVYDKEYLEQISTLINTTDRCLLNNYMIWNLVRKTSSFLDQRFQDADEKFMEVMYGTKKTCLPRWKFCVSDTENNLGFALGPMFVKATFAEDSKSIATEIILEIKKAFEESLSTLKWMDEETRKSAKEKADAIYNMIGYPNFIMDPKELDKVFNDYTAVPDLYFENAMRFFNFSWRVTADQLRKAPNRDQWSMTPPMVNAYYSPTKNEIVFPAGILQAPFYTRSSPKALNFGGIGVVVGHELTHAFDDQGREYDKDGNLRPWWKNSSVEAFKRQTECMVEQYSNYSVNGEPVNGRHTLGENIADNGGLKAAYRAYQNWVKKNGAEHSLPTLGLTNNQLFFLGFAQVWCSVRTPESSHEGLITDPHSPSRFRVIGSLSNSKEFSEHFRCPPGSPMNPPHKCEVW

分子量

94-130 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

ECE-1 Protein, Human (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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