EGFR vIII 蛋白, Human (Biotinylated, HEK293, His-Avi)

The EGFRvIII protein is a transmembrane glycoprotein in the protein kinase superfamily that acts as a receptor for epidermal growth factor. It acts on the cell surface, binds to epidermal growth factor, triggers receptor dimerization and tyrosine autophosphorylation, and promotes cell proliferation. EGFR vIII Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived EGFR vIII protein, expressed by HEK293 , with C-Avi, C-His labeled tag. The total length of EGFR vIII Protein, Human (Biotinylated, HEK293, His-Avi) is 354 a.a., with molecular weight of 65-80 kDa.

EGFRvIII 蛋白是属于蛋白激酶超家族的跨膜糖蛋白，并作为表皮生长因子家族成员的受体。作为细胞表面蛋白，EGFRvIII 与表皮生长因子结合，启动受体二聚化和酪氨酸自磷酸化，从而促进细胞增殖。该基因的突变与肺癌有关。此外，EGFRvIII 被认为是细胞因子风暴的一个组成部分，导致严重急性呼吸综合征冠状病毒-2 (SARS-CoV-2) 感染引起的 2019 年冠状病毒病 (COVID-19) 变得更加严重。[RefSeq 提供，2020 年 7 月]

1.Immobilized Biotinylated Human EGFR vIII His at 0.5 μg/mL (100μL/Well) on the plate. Dose response curve for Anti-EGFR vIII Antibody hFc with the EC₅₀ < 20 ng/mL determined by ELISA.
2.Loaded Anti-Human EGFR mAb on Pro-A Biosensor, can bind EGFR vIII Protein, Human (Biotinylated, HEK293, His-Avi) with an affinity constant of 3.64 nM as determined in BLI assay. 

Human

HEK293

C-Avi;C-His

NP_001333870 (L25-S378)

1956  [NCBI]

LEEKKGNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGIGIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVKEITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISDGDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWGPEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGPDNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEGCPTNGPKIPS

65-80 kDa

Greater than 95% as determined by Tris-Bis PAGE.

无菌粉末

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4 or 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.