EIF4E 蛋白, Human

IF4E protein plays multiple roles in cells, regulating processes such as protein synthesis, mRNA export, RNA processing and splicing. As part of the eIF4F protein complex, IF4E recognizes the mRNA cap and promotes ribosome binding. It is also involved in translation repression and regulation of mRNA stability. In P bodies, IF4E is involved in storing translationally inactive mRNA. In addition, IF4E also plays a role in spermatogenesis, neurogenesis, and mRNA nuclear-cytoplasmic transport. The ability of IF4E to participate in mRNA export relies on binding to the m7G cap and the EIF4E-sensitive element (4ESE). LRPPRC promotes the formation of EIF4E-dependent mRNA export complexes. The action of IF4E changes the composition of nuclear pores and promotes the nuclear export of specific mRNAs. EIF4E Protein, Human is the recombinant human-derived EIF4E protein, expressed by E. coli , with tag free. The total length of EIF4E Protein, Human is 217 a.a., with molecular weight of approximately 28 kDa.

IF4E 蛋白在细胞过程中发挥多方面的作用，协调细胞质和细胞核的重要功能。在细胞质中，它充当蛋白质合成的关键引发剂和调节剂。此外，在细胞核内，IF4E 对于将一部分 mRNA 输出到细胞质、促进 RNA 加帽、加工和剪接等过程是必不可少的。作为 eIF4F 蛋白复合物的组成部分，IF4E 在识别 mRNA 帽、解开 5' 末端二级结构以及将 mRNA 募集到核糖体中发挥着至关重要的作用。它能够结合含有 7-甲基鸟苷 (m7G) 的 mRNA 帽，从而启动蛋白质合成并诱导 mRNA 二级结构解旋，从而促进核糖体结合。IF4E 与 EIF4G1 合作，抵消了 EIF1-EIF4G1 促进的扫描，这对于 TISU 翻译至关重要，其中 TISU 元素识别消除了扫描的需要。除了翻译起始之外，IF4E 还充当翻译和稳定性的细胞质调节因子。它是 CYFIP1-EIF4E-FMR1 复合物的一部分，通过与 mRNA 帽结合来介导翻译抑制。此外，在 P 体中，IF4E 参与储存翻译失活 mRNA 并防止其降解的复合体。其多种功能延伸至精子发生、神经发生和 mRNA 核细胞质运输中的作用。值得注意的是，IF4E 参与 mRNA 输出依赖于其结合 m7G 帽的能力以及 EIF4E 敏感元件 (4ESE) 的存在。LRPPRC 促进与 4ESE 的相互作用，形成 EIF4E 依赖性 mRNA 输出复合物。IF4E 依赖性 mRNA 输出，独立于正在进行的蛋白质或 RNA 合成，是 XPO1 依赖性的，LRPPRC 与 XPO1 相互作用。IF4E 的动态作用改变核孔的组成，通过调节关键因子的表达来促进 RNA 输出。它显着促进特定 mRNA 的核输出，包括细胞周期蛋白 CCND1、NOS2/iNOS 和 MDM2。

Human

E. coli

Tag Free

AAH12611.1 (M1-V217)

1977  [NCBI]

MATVEPETTPTPNPPTTEEEKTESNQEVANPEHYIKHPLQNRWALWFFKNDKSKTWQANLRLISKFDTVEDFWALYNHIQLSSNLMPGCDYSLFKDGIEPMWEDEKNKRGGRWLITLNKQQRRSDLDRFWLETLLCLIGESFDDYSDDVCGAVVNVRAKGDKIAIWTTECENREAVTHIGRVYKERLGLPPKIVIGYQSHADTATKSGSTTKNRFVV

approximately 28 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4 or 50 mM Tris-HCL, 300 mM NaCL, 200 mM arginine, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.