Endothelin-1/EDN1 蛋白, Human (GST)

Endothelin-1/EDN1, an endothelium-derived vasoconstrictor peptide, acts as a likely ligand for G-protein coupled receptors EDNRA and EDNRB. Its binding initiates a signaling cascade in glomerular mesangial cells involving PTK2B, BCAR1, BCAR3, and GTPases RAP1 and RHOA. Furthermore, Endothelin-1/EDN1 shows binding affinity for the DEAR/FBXW7-AS1 receptor, underscoring its involvement in diverse cellular signaling and vascular regulation. Endothelin-1/EDN1 Protein, Human (GST) is the recombinant human-derived Endothelin-1/EDN1 protein, expressed by E. coli , with N-GST labeled tag. The total length of Endothelin-1/EDN1 Protein, Human (GST) is 21 a.a., with molecular weight of 29.5 kDa.

Endothelin-1/EDN1 是一种源自内皮的血管收缩肽，可能是 G 蛋白偶联受体 EDNRA 和 EDNRB 的配体。结合后，它会激活肾小球系膜细胞中涉及 PTK2B、BCAR1、BCAR3 以及 GTPases RAP1 和 RHOA 的信号级联反应。此外，Endothelin-1/EDN1 对 DEAR/FBXW7-AS1 受体具有结合亲和力，有助于其在细胞信号传导和血管调节中发挥多种作用。

1.Measured by its binding ability in a functional ELISA. Immobilized EDNRB at 5 μg/mL can bind human EDN1 with a linear range of 37035-185619 ng/mL.
2.Measured by its binding ability in a functional ELISA. Immobilized EDNRB at 0.5 μg/mL can bind human EDN1 with a linear range of 213.5-245.4 μg/ml.

Human

E. coli

N-GST

P05305 (C53-W73)

1906  [NCBI]

CSCSSLMDKECVYFCHLDIIW

29.5 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, 6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.