1. 重组蛋白
  2. Enzymes & Regulators
  3. Lyases (EC 4)
  4. Enolase 1/ENO1 蛋白, Mouse (His)

Enolase 1/ENO1 蛋白, Mouse (His)

目录号: HY-P72182
COA 产品使用指南

烯醇化酶 1/ENO1 蛋白作为一种糖酵解酶,可将 2-磷酸甘油酸转化为磷酸烯醇丙酮酸,并在生长控制、耐缺氧性和过敏反应等细胞过程中发挥多种作用。它们还在纤溶系统中发挥作用并刺激免疫球蛋白的产生,表明它们参与免疫反应。Enolase 1/ENO1 蛋白, Mouse (His) 是重组的 烯醇化酶 ENO1 蛋白,由 E. coli 表达,带有 N-10*His 标签。Enolase 1/ENO1 蛋白, Mouse (His) 全长 432 个氨基酸,分子量约为 ~50.4 kDa。

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规格 价格 是否有货 数量
20 μg ¥1500 In-stock
50 μg ¥3300 In-stock
100 μg   询价  

* Please select Quantity before adding items.

Enolase 1/ENO1 Protein, Mouse (His) 相关产品:

  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

Enolase 1/ENO1 Proteins, as a glycolytic enzyme, convert 2-phosphoglycerate to phosphoenolpyruvate and have diverse roles in cellular processes like growth control, hypoxia tolerance, and allergic responses. They also function in the fibrinolytic system and stimulate immunoglobulin production, showing their involvement in immune responses. Enolase 1/ENO1 Protein, Mouse (His) is the recombinant mouse-derived Enolase 1/ENO1 protein, expressed by E. coli , with N-10*His labeled tag. The total length of Enolase 1/ENO1 Protein, Mouse (His) is 432 a.a., with molecular weight of ~50.4 kDa.

研究背景

烯醇化酶 1 (ENO1) 是一种糖酵解酶,负责催化 2-磷酸甘油酸转化为磷酸烯醇丙酮酸。除了在糖酵解中的作用之外,ENO1 在各种细胞过程中发挥着多方面的作用,包括生长控制、缺氧耐受性和过敏反应。值得注意的是,它在血管内和细胞周纤溶系统中发挥作用,在细胞表面充当纤溶酶原的受体和激活剂,在白细胞和神经元等多种细胞类型中观察到。此外,ENO1 具有刺激免疫球蛋白产生的能力,进一步强调了它参与免疫反应。

生物活性

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

种属

Mouse

表达系统

E. coli

标签

N-10*His

蛋白编号

P17182 (S2-A433)

基因 ID
蛋白结构
N-term
10*His
ENO1 (S2-A433)
Accession # P17182
C-term
同用名
Eno1; Eno-1; Alpha-enolase; EC 4.2.1.11; 2-phospho-D-glycerate hydro-lyase; Enolase 1; Non-neural enolase; NNE
氨基酸序列

SILRIHAREIFDSRGNPTVEVDLYTAKGLFRAAVPSGASTGIYEALELRDNDKTRFMGKGVSQAVEHINKTIAPALVSKKVNVVEQEKIDKLMIEMDGTENKSKFGANAILGVSLAVCKAGAVEKGVPLYRHIADLAGNPEVILPVPAFNVINGGSHAGNKLAMQEFMILPVGASSFREAMRIGAEVYHNLKNVIKEKYGKDATNVGDEGGFAPNILENKEALELLKTAIAKAGYTDQVVIGMDVAASEFYRSGKYDLDFKSPDDPSRYITPDQLADLYKSFVQNYPVVSIEDPFDQDDWGAWQKFTASAGIQVVGDDLTVTNPKRIAKAASEKSCNCLLLKVNQIGSVTESLQACKLAQSNGWGVMVSHRSGETEDTFIADLVVGLCTGQIKTGAPCRSERLAKYNQILRIEEELGSKAKFAGRSFRNPLA

分子量

Approximately 50.4 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

性状

无菌粉末

组分

Lyophilized from a 0.2 μm solution of 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

Enolase 1/ENO1 Protein, Mouse (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Enolase 1/ENO1 Protein, Mouse (His)
目录号:
HY-P72182
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