Enolase 3/ENO3 蛋白, Human (His)

Enolase 3/ENO3 protein acts as a glycolytic enzyme, promoting the conversion of 2-phosphoglycerate into phosphoenolpyruvate. In addition to its role in glycolysis, ENO3 appears to play a crucial role in the development and regeneration of striated muscle. Enolase 3/ENO3 Protein, Human (His) is the recombinant human-derived Enolase 3/ENO3 protein, expressed by E. coli , with N-His labeled tag.

烯醇化酶 3 (ENO3) 是一种糖酵解酶，负责在糖酵解途径中催化 2-磷酸甘油酸转化为磷酸烯醇丙酮酸。除了在能量代谢中的经典作用外，ENO3 似乎还在横纹肌的发育和再生中发挥着特定的功能。该酶参与这些过程表明其在支持肌肉组织发育和修复过程中的能量需求方面发挥着作用。这种双重功能凸显了 ENO3 在基本代谢途径和与肌肉发育和再生相关的特殊细胞过程中的多方面作用。

Specific activity is 5235 pmol/min/µg, and was obtained by measuring the decrease of NAD that incubate at 37 ºC for 5 minutes in absorbance at 340 nm resulting from NADH at pH 6.5.

Human

E. coli

N-10*His

P13929-1/AAH17249.1 (M1-K434)

2027  [NCBI]

MAMQKIFAREILDSRGNPTVEVDLHTAKGRFRAAVPSGASTGIYEALELRDGDKGRYLGKGVLKAVENINNTLGPALLQKKLSVVDQEKVDKFMIELDGTENKSKFGANAILGVSLAVCKAGAAEKGVPLYRHIADLAGNPDLILPVPAFNVINGGSHAGNKLAMQEFMILPVGASSFKEAMRIGAEVYHHLKGVIKAKYGKDATNVGDEGGFAPNILENNEALELLKTAIQAAGYPDKVVIGMDVAASEFYRNGKYDLDFKSPDDPARHITGEKLGELYKSFIKNYPVVSIEDPFDQDDWATWTSFLSGVNIQIVGDDLTVTNPKRIAQAVEKKACNCLLLKVNQIGSVTESIQACKLAQSNGWGVMVSHRSGETEDTFIADLVVGLCTGQIKTGAPCRSERLAKYNQLMRIEEALGDKAIFAGRKFRNPKAK

Approximately 50 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.