ENPP-1 蛋白, Mouse (HEK293, His)

ENPP-1 is a nucleotide pyrophosphatase that critically regulates bone mineralization and soft tissue calcification through the production of diphosphate (PPi).This PPi inhibits the growth of hydroxyapatite crystals and prevents unwanted mineralization.ENPP-1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived ENPP-1 protein, expressed by HEK293 , with C-His labeled tag.

ENPP-1 蛋白是一种核苷酸焦磷酸酶，通过调节焦磷酸盐水平在骨矿化和软组织钙化中发挥至关重要的作用。它产生二磷酸盐 (PPi)，通过与新生的羟基磷灰石晶体结合，防止其进一步生长，从而抑制骨骼的矿化和软组织的钙化。ENPP-1 表现出优先水解 ATP，但也可以水解其他核苷 5' 三磷酸和二腺苷多磷酸。此外，它还参与内质网和高尔基体中核苷酸糖的调节，影响嘌呤能信号传导。该蛋白通过抑制hedgehog信号传导来抑制异位关节钙化并维持关节软骨细胞，尽管hedgehog抑制的直接或间接性质仍不清楚。ENPP-1 参与调节胰岛素敏感性和黑素生成。值得注意的是，它可以水解 2',3'-cGAMP，这是激活 TMEM173/STING 并触发 I 型干扰素产生的第二信使，尽管这种水解在细胞外空间中的确切作用尚不完全清楚。然而，ENPP-1 无法水解 2',3'-cGAMP 连接异构体 3',3'-cGAMP。

Measured by its ability to hydrolyze thymidine 5'-monophosphate p-nitrophenyl ester. The specific activity is >100000 pmol/min/μg, as measured under the described conditions.

Mouse

HEK293

C-His

P06802-1 (K80-D906)

18605  [NCBI]

KPSCAKEVKSCKGRCFERTFSNCRCDAACVSLGNCCLDFQETCVEPTHIWTCNKFRCGEKRLSRFVCSCADDCKTHNDCCINYSSVCQDKKSWVEETCESIDTPECPAEFESPPTLLFSLDGFRAEYLHTWGGLLPVISKLKNCGTYTKNMRPMYPTKTFPNHYSIVTGLYPESHGIIDNKMYDPKMNASFSLKSKEKFNPLWYKGQPIWVTANHQEVKSGTYFWPGSDVEIDGILPDIYKVYNGSVPFEERILAVLEWLQLPSHERPHFYTLYLEEPDSSGHSHGPVSSEVIKALQKVDRLVGMLMDGLKDLGLDKCLNLILISDHGMEQGSCKKYVYLNKYLGDVNNVKVVYGPAARLRPTDVPETYYSFNYEALAKNLSCREPNQHFRPYLKPFLPKRLHFAKSDRIEPLTFYLDPQWQLALNPSERKYCGSGFHGSDNLFSNMQALFIGYGPAFKHGAEVDSFENIEVYNLMCDLLGLIPAPNNGSHGSLNHLLKKPIYNPSHPKEEGFLSQCPIKSTSNDLGCTCDPWIVPIKDFEKQLNLTTEDVDDIYHMTVPYGRPRILLKQHHVCLLQQQQFLTGYSLDLLMPLWASYTFLRNDQFSRDDFSNCLYQDLRIPLSPVHKCSYYKSNSKLSYGFLTPPRLNRVSNHIYSEALLTSNIVPMYQSFQVIWHYLHDTLLQRYAHERNGINVVSGPVFDFDYDGRYDSLEILKQNSRVIRSQEILIPTHFFIVLTSCKQLSETPLECSALESSAYILPHRPDNIESCTHGKRESSWVEELLTLHRARVTDVELITGLSFYQDRQESVSELLRLKTHLPIFSQED

100-110 kDa

Greater than 95% as determined by Tris-Bis PAGE.

无菌粉末

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 8% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.