ENPP-2 蛋白, Human (HEK293, His)

The ENPP-2 protein catalyzes the hydrolysis of lysophospholipids (especially lysophosphatidylcholine) to produce the signaling molecule lysophosphatidic acid (LPA). It also acts on sphingosine phosphocholine to produce sphingosine 1-phosphate, which affects cell movement. ENPP-2 Protein, Human (HEK293, His) is the recombinant human-derived ENPP-2 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of ENPP-2 Protein, Human (HEK293, His) is 828 a.a., with molecular weight of 100-120 kDa.

ENPP-2 蛋白水解溶血磷脂，特别是溶血磷脂酰胆碱，在细胞外液中产生信号分子溶血磷脂酸 (LPA)。它还可以作用于鞘氨醇磷酸胆碱，产生 1-磷酸鞘氨醇，调节细胞运动。此外，ENPP-2 蛋白已被证明可以不同程度地水解 bis-pNPP、pNP-TMP 和 ATP。它参与各种运动相关过程，包括血管生成和神经突生长，并通过刺激平滑肌细胞的迁移和微管形成而充当血管生成因子。ENPP-2 蛋白还可能通过百日咳毒素敏感的 G 蛋白参与黑色素瘤细胞迁移。它可能在诱导分娩、细胞增殖和脂肪组织发育中发挥作用。此外，活化血小板中 LPA 的产生以及裂解 sn-1 溶血磷脂以产生主要含有 18:2 和 20:4 脂肪酸的 sn-1 溶血磷脂酸是必需的。ENPP-2 蛋白优先选择 1-O-烷基-sn-甘油-3-磷酸胆碱 (lyso-PAF) 的 sn-1 异构体。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

HEK293

C-6*His

AAH34961.1 (A36-I863)

5168  [NCBI]

AEGWEEGPPTVLSDSPWTNISGSCKGRCFELQEAGPPDCRCDNLCKSYTSCCHDFDELCLKTARGWECTKDRCGEVRNEENACHCSEDCLARGDCCTNYQVVCKGESHWVDDDCEEIKAAECPAGFVRPPLIIFSVDGFRASYMKKGSKVMPNIEKLRSCGTHSPYMRPVYPTKTFPNLYTLATGLYPESHGIVGNSMYDPVFDATFHLRGREKFNHRWWGGQPLWITATKQGVKAGTFFWSVVIPHERRILTILQWLTLPDHERPSVYAFYSEQPDFSGHKYGPFGPEMTNPLREIDKIVGQLMDGLKQLKLHRCVNVIFVGDHGMEDVTCDRTEFLSNYLTNVDDITLVPGTLGRIRSKFSNNAKYDPKAIIANLTCKKPDQHFKPYLKQHLPKRLHYANNRRIEDIHLLVERRWHVARKPLDVYKKPSGKCFFQGDHGFDNKVNSMQTVFVGYGSTFKYKTKVPPFENIELYNVMCDLLGLKPAPNNGTHGSLNHLLRTNTFRPTMPEEVTRPNYPGIMYLQSDFDLGCTCDDKVEPKNKLDELNKRLHTKGSTEERHLLYGRPAVLYRTRYDILYHTDFESGYSEIFLMPLWTSYTVSKQAEVSSVPDHLTSCVRPDVRVSPSFSQNCLAYKNDKQMSYGFLFPPYLSSSPEAKYDAFLVTNMVPMYPAFKRVWNYFQRVLVKKYASERNGVNVISGPIFDYDYDGLHDTEDKIKQYVEGSSIPVPTHYYSIITSCLDFTQPADKCDGPLSVSSFILPHRPDNEESCNSSEDESKWVEELMKMHTARVRDIEHLTSLDFFRKTSRSYPEILTLKTYLHTYESEI

100-120 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.