ENPP-3 蛋白, Mouse (HEK293, His)

ENPP-3 is a hydrolase that metabolizes extracellular nucleotides (ATP, GTP, UTP, CTP), which is critical for immune regulation of inflammation and chronic allergic reactions. By eliminating extracellular ATP, ENPP-3 inhibits basophil and mast cell activation and suppresses the release of inflammatory cytokines. ENPP-3 Protein, Mouse (HEK293, His) is the recombinant mouse-derived ENPP-3 protein, expressed by HEK293 , with N-10*His labeled tag. The total length of ENPP-3 Protein, Mouse (HEK293, His) is 829 a.a., with molecular weight of 100-120 kDa.

ENPP-3 是一种水解酶，具有代谢各种细胞外核苷酸的能力，包括 ATP、GTP、UTP 和 CTP。这种酶活性在调节免疫反应中发挥着至关重要的作用，特别是在炎症和过敏反应的慢性阶段。ENPP-3 通过消除细胞外 ATP 发挥作用，ATP 是一种激活嗜碱性粒细胞和肥大细胞的信号分子，导致炎症细胞因子的释放。此外，在小肠腔内，ENPP-3 代谢细胞外 ATP，从而防止 ATP 诱导的肠浆细胞样树突状细胞凋亡。除了参与核苷酸代谢外，ENPP-3 还表现出碱性磷酸二酯酶活性，增加了其在细胞过程中的多方面作用。

Immobilized  Human ENPP3 at 2 μg/mL (100 μL/well) can bind Anti-ENPP3 Antibody, The ED₅₀  for this effect is 5.644 ng/mL.

Mouse

HEK293

N-10*His

Q6DYE8-1 (R46-I874)

209558  [NCBI]

RKPEEQGSCRKKCFDSSHRGLEGCRCDSGCTGRGDCCWDFEDTCVKSTQIWTCNLFRCGENRLETALCSCADDCLQRKDCCADYKTVCQGESPWVTEACASSQEPQCPPGFDLPPVILFSMDGFRAEYLQTWSTLLPNINKLKTCGIHSKYMRAMYPTKTFPNHYTIVTGLYPESHGIIDNNMYDVHLNKNFSLSSVEKSNPAWWSGQPIWLTAMYQGLKAACYYWPGSDVAVNGSFPTIYRNYSNSVPYERRITTLLQWLDLPKADRPSFYTIYVEEPDSAGHSSGPVSAGVIKALQSVDNAFGMLMEGLKQRNLHNCVNIIVLADHGMDQTSCDRVEYMTDYFPKINFYMYQGPAPRIRTRNIPQDFFTFNSEEIVRNLSCRKPDQHFKPYLTPDLPKRLHYAKNVRIDKAHLMVDRQWLAFRSKGSSNCGGGTHGYNNEFKSMEAIFLAHGPSFIEKTVIEPFENIEVYNLLCDLLHIEPAPNNGTHGSLNHLLKTPFYKPSHAGELSTPADCGFTTPLPTDPLDCSCPALQNTPGLEEQANQRLNLSEGEVAATVKANLPFGRPRVMQKNGDHCLLYHRDYISGYGKAMKMPMWSSYTVLKPGDTSSLPPTVPDCLRADVRVAPSESQKCSFYLADKNITHGFLYPAIKGTNESRYDALITSNLVPMYKEFKKMWDYFHEVLLIKYAIERNGLNVVSGPIFDYNYDGHFDAPDEITQYVAGTDVPIPTHYFVVLTSCKDQTHTPDSCPGWLDVLPFIVPHRPTNIESCSENKTEDLWVEERFQAHAARVRDVELLTGLDFYQEKAQPVSQILQLKTYLPTFETII

100-120 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized a 0.22 μm filtered solution of 50 mM Tris, 150 mM NaCl, pH 7.5.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.