ENPP-5 蛋白, Human (HEK293, His)

ENPP-5, also known as ectonucleotide pyrophosphatase/phosphodiesterase 5, has the ability to hydrolyze nicotinamide adenine dinucleotide (NAD) but does not have lysophospholipase D activity, indicating its inability to hydrolyze Nucleotide diphosphates and triphosphates. This enzymatic activity suggests that ENPP-5 may play a role in regulating NAD levels and possibly affecting cellular processes involved in NAD metabolism. ENPP-5 Protein, Human (HEK293, His) is the recombinant human-derived ENPP-5 protein, expressed by HEK293 , with C-His labeled tag.

ENPP-5，也称为外核苷酸焦磷酸酶/磷酸二酯酶 5，具有水解烟酰胺腺嘌呤二核苷酸 (NAD) 的能力，但不具有溶血磷脂酶 D 活性，表明其无法水解核苷酸二磷酸和三磷酸。这种酶活性表明 ENPP-5 可能在调节 NAD 水平并可能影响涉及 NAD 代谢的细胞过程中发挥作用。此外，该酶在神经元细胞中的存在表明它可能通过其酶特性促进神经元细胞通讯。需要进一步的研究来充分了解 ENPP-5 的具体功能和调节机制，以及它在神经元信号传导和细胞稳态中的潜在影响。

Measured by its ability to cleave 2 mM O-(4-Nitrophenylphosphoryl) choline that at room temperature for 30 minutes. The specific activity is 303.591 pmol/min/μg.

Human

HEK293

C-10*His

Q9UJA9 (P25-G429)

59084  [NCBI]

PDQQKVLLVSFDGFRWDYLYKVPTPHFHYIMKYGVHVKQVTNVFITKTYPNHYTLVTGLFAENHGIVANDMFDPIRNKSFSLDHMNIYDSKFWEEATPIWITNQRAGHTSGAAMWPGTDVKIHKRFPTHYMPYNESVSFEDRVAKIIEWFTSKEPINLGLLYWEDPDDMGHHLGPDSPLMGPVISDIDKKLGYLIQMLKKAKLWNTLNLIITSDHGMTQCSEERLIELDQYLDKDHYTLIDQSPVAAILPKEGKFDEVYEALTHAHPNLTVYKKEDVPERWHYKYNSRIQPIIAVADEGWHILQNKSDDFLLGNHGYDNALADMHPIFLAHGPAFRKNFSKEAMNSTDLYPLLCHLLNITAMPHNGSFWNVQDLLNSAMPRVVPYTQSTILLPGSVKPAEYDQEG

Approximately 68-95 kDa due to the glycosylation.

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.