Enterotoxin type B 蛋白, S. aureus (P.pastoris, His)

Enterotoxins (SEBs) activate the host immune system by binding raw molecules to major histocompatibility complex class II and T-cell receptor (TCR) molecules. The formation of the ternary complex triggers significant activation of T lymphocytes, leading to the systemic release of pro-inflammatory cytokines. Enterotoxin type B Protein, S. aureus (P.pastoris, His) is the recombinant Staphylococcus aureus-derived Enterotoxin type B protein, expressed by P. pastoris , with N-6*His labeled tag. The total length of Enterotoxin type B Protein, S. aureus (P.pastoris, His) is 239 a.a., with molecular weight of ~30.4 kDa.

E 型肠毒素 (SEB) 是一种葡萄球菌肠毒素，通过以未加工的分子形式与主要组织相容性复合物 II 类和 T 细胞受体 (TCR) 分子结合来激活宿主免疫系统。这种三元复合物的形成导致大量 T 淋巴细胞的激活，启动促炎细胞因子的全身释放。此外，SEB 还与葡萄球菌食物中毒综合征的发生有关。SEB 与由 α/HLA-DRA 和 β/HLA-DRB1 链组成的 MHC II 类分子以及 T 细胞受体 β 变量 19/TRBV19 发生特异性相互作用。这些相互作用强调了其在免疫系统激活和相关病理反应中的作用。

Staphylococcus aureus

P. pastoris

N-6*His

P01552 (E28-K266)

/

ESQPDPKPDELHKSSKFTGLMENMKVLYDDNHVSAINVKSIDQFLYFDLIYSIKDTKLGNYDNVRVEFKNKDLADKYKDKYVDVFGANYYYQCYFSKKTNDINSHQTDKRKTCMYGGVTEHNGNQLDKYRSITVRVFEDGKNLLSFDVQTNKKKVTAQELDYLTRHYLVKNKKLYEFNNSPYETGYIKFIENENSFWYDMMPAPGDKFDQSKYLMMYNDNKMVDSKDVKIEVYLTTKKK

Approximately 30.4 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized after extensive dialysis against solution in 20 mM Tris-HC1, 0.5 M NaCl, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.