1. 重组蛋白
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  4. Enterotoxin type C-2 蛋白, S. aureus

Enterotoxin type C-2 蛋白, S. aureus

目录号: HY-P71465
产品使用指南

C-2 型肠毒素 (SEC2) 通过将未加工的分子与主要组织相容性复合物 II 类和 T 细胞受体 (TCR) 分子结合来激活宿主免疫系统。这种相互作用形成三元复合物,激活大量 T 淋巴细胞并引发促炎细胞因子的广泛释放。Enterotoxin type C-2 蛋白, S. aureus 是重组的 C-2 型肠毒素蛋白,由 E. coli 表达,不带标签。Enterotoxin type C-2 蛋白, S. aureus 全长 239 个氨基酸,分子量约为 ~27.6 kDa。

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描述

Enterotoxin type C-2 (SEC2) activates the host immune system by binding the unprocessed molecule to major histocompatibility complex class II and T-cell receptor (TCR) molecules. This interaction forms a ternary complex that activates a large number of T lymphocytes and triggers the widespread release of proinflammatory cytokines. Enterotoxin type C-2 Protein, S. aureus is the recombinant Staphylococcus aureus-derived Enterotoxin type C-2 protein, expressed by E. coli , with tag free. The total length of Enterotoxin type C-2 Protein, S. aureus is 239 a.a., with molecular weight of ~27.6 kDa.

研究背景

C-2 型肠毒素 (SEC2) 是一种葡萄球菌肠毒素,通过以未加工的分子形式与主要组织相容性复合物 II 类和 T 细胞受体 (TCR) 分子结合来激活宿主免疫系统。这种相互作用形成三元复合物,随后激活大量 T 淋巴细胞,引发促炎细胞因子的广泛释放。此外,SEC2 还与葡萄球菌食物中毒综合征的发生有关,该综合征的特征是胃肠道不适和全身性疾病等症状。

种属

Staphylococcus aureus

表达系统

E. coli

标签

Tag Free

蛋白编号

P34071 (E28-G266)

基因 ID

/

蛋白结构
N-term
SEC2 (E28-G266)
Accession # P34071
C-term
同用名
entC2; Enterotoxin type C-2; SEC2
氨基酸序列

ESQPDPTPDELHKSSEFTGTMGNMKYLYDDHYVSATKVMSVDKFLAHDLIYNISDKKLKNYDKVKTELLNEDLAKKYKDEVVDVYGSNYYVNCYFSSKDNVGKVTGGKTCMYGGITKHEGNHFDNGNLQNVLIRVYENKRNTISFEVQTDKKSVTAQELDIKARNFLINKKNLYEFNSSPYETGYIKFIENNGNTFWYDMMPAPGDKFDQSKYLMMYNDNKTVDSKSVKIEVHLTTKNG

分子量

Approximately 27.6 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

Enterotoxin type C-2 Protein, S. aureus 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Enterotoxin type C-2 Protein, S. aureus
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HY-P71465
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