1. 重组蛋白
  2. Viral Proteins
  3. envelope glycoprotein gp64 蛋白, AcmNPV (HEK293, His)

envelope glycoprotein gp64 蛋白, AcmNPV (HEK293, His)

目录号: HY-P76711
产品使用指南

包膜糖蛋白 gp64 被归类为 III 类病毒融合蛋白。作为一种包膜磷酸糖蛋白,它促进病毒与宿主内体膜的融合,从而促进病毒进入宿主细胞。在受体介导的病毒内化之后,gp64 在低 pH 下经历构象变化,进入可融合状态,从而在细胞融合时将核衣壳释放到宿主细胞的细胞质中。envelope glycoprotein gp64 蛋白, AcmNPV (HEK293, His) 是重组的 包膜糖蛋白 gp64 蛋白,由 HEK293 表达,带有 C-His 标签。

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描述

Envelope glycoprotein gp64 is classified as a class III viral fusion protein. As an envelope phosphate glycoprotein, it promotes the fusion of virus and host endosomal membranes, thereby promoting virus entry into host cells. Following receptor-mediated viral internalization, gp64 undergoes a conformational change at low pH into a fusion-competent state, thereby releasing the nucleocapsid into the host cell cytoplasm upon cell fusion. envelope glycoprotein gp64 Protein, AcmNPV (HEK293, His) is the recombinant Virus-derived envelope glycoprotein gp64 protein, expressed by HEK293 , with C-His labeled tag.

研究背景

包膜糖蛋白 gp64 是一种 III 类病毒融合蛋白,也是某些病毒的关键成分。作为一种包膜磷酸糖蛋白,gp64 在介导病毒与宿主内体膜之间的融合、促进病毒进入宿主细胞方面发挥着关键作用。在受体介导的内化过程中,gp64 在低 pH 条件下发生构象变化,进入可融合状态,从而在细胞融合后将病毒核衣壳释放到细胞质中。此外,gp64 可能参与出芽过程。在结构上,gp64 形成二硫键连接的同源三聚体,有助于其在病毒融合过程中的稳定性和功能性(参考:未提供具体参考)。

种属

Virus

表达系统

HEK293

标签

C-His

蛋白编号

P17501/NP_054158.1 (A21-T481)

基因 ID

1403961  [NCBI]

蛋白结构
N-term
AcmNPV gp64 (A21-T481)
Accession # P17501/NP_054158.1
His
C-term
同用名
Major envelope glycoprotein; GP64; GP67; AcmNPV-gp64
分子量

Approximately 54.2 kDa.

纯度

Greater than 95% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

envelope glycoprotein gp64 Protein, AcmNPV (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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envelope glycoprotein gp64 Protein, AcmNPV (HEK293, His)
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HY-P76711
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