1. 重组蛋白
  2. Cytokines and Growth Factors
  3. Chemokine & Receptors
  4. CC Chemokines
  5. Eotaxin/CCL11
  6. Eotaxin/CCL11 蛋白, Human

Eotaxin/CCL11 蛋白, Human

目录号: HY-P7159
COA 产品使用指南

Eotaxin/CCL11 蛋白, Human是嗜酸性粒细胞、嗜碱性粒细胞和Th2淋巴细胞的有效化学诱导剂。

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规格 价格 是否有货 数量
10 μg ¥1000 In-stock
50 μg ¥2800 In-stock
100 μg   询价  

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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

  • 参考文献

描述

Eotaxin/CCL11 Protein, Human is a potent chemoattractant for eosinophil cells and provides a new mechanism to explain tissue eosinophilia.

研究背景

CCL11 是属于 CC 趋化因子家族的一种小细胞因子。CCL11 通过诱导嗜酸性粒细胞的趋化性选择性地募集嗜酸性粒细胞,因此与过敏反应有关[1]。它由 IFN-γ 刺激的内皮细胞和 TNF 激活的单核细胞产生。CCL11 是嗜酸性粒细胞、嗜碱性粒细胞和 Th2 淋巴细胞的有效化学引诱剂。CCL11 在疾病过程中具有许多重要的生物学功能。它在过敏性和非过敏性炎症反应(例如分枝杆菌和血吸虫引起的肉芽肿病)中发挥着关键作用[2]

体外研究
(In Vitro)

CCL11 (0.1-100 nM, 48 h) can induce HMEC chemotaxis in a dose-dependent manner and can be blocked by 20 μg/mL of CCL11 mAb. However, CCL11 does not induce HMEC proliferation in vitro[3].
CCL11 (1-100 ng) can induce angiogenesis, with 78% at 100 ng and 63% at 10 ng but no significant angiogenic response was obtained at a dose of 1 ng in the chick CAM assay[3].

生物活性

Full biological activity determined by a chemotaxis bioassay using human peripheral blood eosinophils is in a concentration range of 0.1-10.0 ng/ml.

种属

Human

表达系统

E. coli

标签

Tag Free

蛋白编号

P51671 (G24-P97)

基因 ID
蛋白结构
N-term
CCL11 (G24-P97)
Accession # P51671
C-term
中文名
重组人嗜酸粒细胞趋化因子 (CCL11)
同用名
rHuEotaxin/CCL11; C-C motif chemokine 11; Eosinophil chemotactic protein; SCYA11
氨基酸序列

GPASVPTTCCFNLANRKIPLQRLESYRRITSGKCPQKAVIFKTKLAKDICADPKKKWVQDSMKYLDQKSPTPKP

分子量

Approximately 8.4 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized after extensive dialysis against 20 mM PB, pH 7.4, 150 mM NaCl.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Eotaxin/CCL11 Protein, Human
目录号:
HY-P7159
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