EpCAM/TROP1 蛋白, Human (HEK293, His)

The EpCAM/TROP1 protein serves as an important homogeneous interacting molecule that promotes direct contact between intestinal epithelial cells (IEC) and intraepithelial lymphocytes (IEL) in the mucosal epithelium. This feature helps establish an immune barrier against mucosal infections. EpCAM/TROP1 Protein, Human (HEK293, His) is the recombinant human-derived EpCAM/TROP1 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of EpCAM/TROP1 Protein, Human (HEK293, His) is 242 a.a., with molecular weight of approximately 34-42 kDa.

EpCAM/TROP1 蛋白作为一个关键实体出现，有可能充当物理同源相互作用分子，促进粘膜上皮的肠上皮细胞 (IEC) 和上皮内淋巴细胞 (IEL) 之间的直接接触，从而有助于建立免疫屏障作为抵抗粘膜感染的主要防御措施。除了在粘膜免疫中的作用之外，这种蛋白质在胚胎干细胞的增殖和分化中也发挥着重要作用。它还通过上调 FABP5、MYC 以及细胞周期蛋白 A 和 E 的表达来进一步表现出调节影响，表明 EpCAM/TROP1 参与关键细胞过程的调节。它的单体性质以及与磷酸化 CLDN7 的相互作用强调了所涉及的复杂的分子相互作用，为该蛋白质在细胞生理学中的多种功能提供了见解。

Measured by the ability of the immobilized protein to support the adhesion of the L929 cells mouse fibroblast cell line. The ED₅₀ for this effect is 0.7677 μg/mL, corresponding to a specific activity is 1.3×10³ units/mg.

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  Measured by the ability of the immobilized protein to support the adhesion of the L929 cells mouse fibroblast cell line. The ED₅₀ for this effect is 0.7677 μg/mL, corresponding to a specific activity is 1.3×10³ units/mg.

Human

HEK293

C-6*His

AAH14785.1 (Q24-K265)

4072  [NCBI]

QEECVCENYKLAVNCFVNNNRQCQCTSVGAQNTVICSKLAAKCLVMKAEMNGSKLGRRAKPEGALQNNDGLYDPDCDESGLFKAKQCNGTSMCWCVNTAGVRRTDKDTEITCSERVRTYWIIIELKHKAREKPYDSKSLRTALQKEITTRYQLDPKFITSILYENNVITIDLVQNSSQKTQNDVDIADVAYYFEKDVKGESLFHSKKMDLTVNGEQLDLDPGQTLIYYVDEKAPEFSMQGLK

approximately 34-42 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2-7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.