1. 重组蛋白
  2. Cytokines and Growth Factors Receptor Proteins Enzymes & Regulators
  3. Ephrin/Eph Family Receptor Tyrosine Kinases Protein Tyrosine Kinases
  4. Eph Receptors
  5. EphA7
  6. EphA7 蛋白, Human (sf9)

EphA7 蛋白, Human (sf9)

目录号: HY-P702708
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EphA7 蛋白是一种受体酪氨酸激酶,它与 GPI 锚定的肝配蛋白-A 配体混杂结合以启动双向信号传导。它参与正向和反向信号传导通路,使用 EFNA5 作为功能配体,调节大脑发育并影响细胞粘附和排斥。EphA7 蛋白, Human (sf9) 是重组的 EphA7 蛋白,由 Sf9 insect cells 表达,带有 tag Free 标签。EphA7 蛋白, Human (sf9) 全长 419 个氨基酸

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描述

The EphA7 protein is a receptor tyrosine kinase that promiscuously binds to the GPI-anchored ephrin-A ligand to initiate bidirectional signaling. It participates in forward and reverse signaling pathways, uses EFNA5 as a functional ligand, regulates brain development and affects cell adhesion and repulsion. EphA7 Protein, Human (sf9) is the recombinant human-derived EphA7, expressed by Sf9 insect cells , with tag Free labeled tag. The total length of EphA7 Protein, Human (sf9) is 419 a.a.,

研究背景

EphA7 蛋白是一种受体酪氨酸激酶,与邻近细胞上 GPI 锚定的肝配蛋白 A 家族配体混杂结合,启动接触依赖性双向信号传导。源自受体的下游途径称为正向信号传导,而肝配蛋白配体下游的途径称为反向信号传导。在 GPI 锚定的肝配蛋白-A 配体中,EFNA5 作为 EPHA7 的同源/功能配体,调节大脑发育并调节细胞间粘附和排斥。EphA7 对轴突表现出排斥活性,在引导皮质丘脑轴突和确保视网膜轴突到丘的正确地形映射方面发挥着至关重要的作用。此外,EphA7 可能通过半胱天冬酶 (CASP3) 依赖性促凋亡活性促进大脑发育。通过 EphA7 的正向信号传导可能会导致 ERK 信号传导通路成分的激活,包括 MAP2K1、MAP2K2、MAPK1 和 MAPK3,这些成分在 EphA7 激活时被磷酸化。

种属

Human

表达系统

Sf9 insect cells

标签

Tag Free

蛋白编号

Q15375-1 (R580-V998)

基因 ID

2045

同用名
EPHA7; EPH receptor A7; EphA7; ephrin type-A receptor 7; Hek11; EK11; EHK-3; EPH-like kinase 11; EPH homology kinase 3; Eph homology kinase-3; receptor protein-tyrosine kinase HEK11; tyrosine-protein kinase receptor EHK-3; EHK3; HEK11
纯度

Greater than 80 % as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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EphA7 Protein, Human (sf9)
目录号:
HY-P702708
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