Ephrin A5 蛋白, Canine (HEK293, Fc)

Ephrin-A5/EFNA5 Protein is a member of the ephrin family of proteins, which are transmembrane proteins that act as both ligands and receptors. It interacts with Eph receptors on adjacent cells through bidirectional signaling, which triggers a series of intracellular events that regulate various cellular processes, including cell adhesion, repulsion, and migration. Ephrin-A5/EFNA5 Protein plays a crucial role in the development of neurons, blood vessels, epithelia, embryonic development, and fertility. Ephrin A5 Protein, Canine (HEK293, Fc) is the recombinant canine-derived Ephrin A5 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of Ephrin A5 Protein, Canine (HEK293, Fc) is 183 a.a., with molecular weight of ~59 kDa.

Ephrin-A5/EFNA5 蛋白是肝配蛋白家族成员，在发育、癌症和许多不同组织的正常功能期间调节细胞形状和粘附，在血管和上皮发育发挥着至关重要的作用。Ephrin-A5/EFNA5 蛋白是神经元细胞引导基因，与神经元发育有关，有助于轴突引导和突触可塑性。Ephrin-A5/EFNA5 蛋白是最佳生育能力和对促性腺激素的完全排卵反应所必需的，参与调节生育^[1]。
Ephrin-A5/EFNA5 Protein 在胚胎发育过程中普遍在多种细胞类型和器官中表达，并调节广泛的早期发育过程，包括组织重塑、骨骼和心脏发育、轴突引导、血管形成和细胞凋亡。Ephrin-A5/EFNA5 Protein 调节卵巢颗粒细胞 (GC) 细胞凋亡、增殖、细胞周期进程、卵母细胞发育和类固醇生成^[2]。

Measured by its binding ability in a functional ELISA. When Recombinant Human EphA3 is immobilized at 10 µg/mL (100 µL/well) can bind Recombinant Canine Ephrin A5. The ED₅₀ for this effect is 10.19 ng/mL.

• 
  Measured by its binding ability in a functional ELISA. When Recombinant Human EphA3 is immobilized at 10 µg/mL (100 µL/well) can bind Recombinant Canine Ephrin A5. The ED₅₀ for this effect is 10.19 ng/mL.

Canine

HEK293

C-hFc

XP_850582 (Q21-N203)

608458  [NCBI]

QDPGSKAVADRYAVYWNSSNPRFQRGDYHIDVCINDYLDVFCPHYEDSVPEDKTERYVLYMVNFDGYSACDHTSKGFKRWECNRPHSPNGPLKFSEKFQLFTPFSLGFEFRPGREYFYISSAIPDNGRRSCLKLKVFVRPTNSCMKTIGVHDRVFDVNDKVENSLEPADDTVHESAEPSRGEN

Approximately 59 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.