1. 重组蛋白
  2. Cytokines and Growth Factors
  3. Ephrin/Eph Family
  4. Ephrins
  5. Ephrin-B1
  6. Ephrin-B1/EFNB1 蛋白, Human (HEK293, His)

Ephrin-B1/EFNB1 蛋白, Human (HEK293, His)

目录号: HY-P70402
COA 产品使用指南

Ephrin-B1/EFNB1 蛋白是一种跨膜配体,与 Eph 受体相互作用,促进双向信号传导。它结合 EPHB1/ELK 和 EPHB2/3,诱导轴突塌陷和生长锥定向。EFNB1 还与 GRIP1/2、TLE1 相互作用,并增强 ZHX2 的转录抑制活性。这些相互作用凸显了 EFNB1 在不同发育过程中的作用。Ephrin-B1/EFNB1 蛋白, Human (HEK293, His) 是重组的 Ephrin-B1/EFNB1 蛋白,由 HEK293 表达,带有 C-6*His 标签。Ephrin-B1/EFNB1 蛋白, Human (HEK293, His) 全长 205 个氨基酸,分子量约为 ~34.0 kDa。

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描述

Ephrin-B1/EFNB1 Protein, a transmembrane ligand, interacts with Eph receptors, facilitating bidirectional signaling. It binds EPHB1/ELK and EPHB2/3, inducing axon collapse and growth cone orientation. EFNB1 also interacts with GRIP1/2, TLE1, and enhances ZHX2's transcriptional repression activity. These interactions highlight EFNB1's role in diverse developmental processes. Ephrin-B1/EFNB1 Protein, Human (HEK293, His) is the recombinant human-derived Ephrin-B1/EFNB1 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Ephrin-B1/EFNB1 Protein, Human (HEK293, His) is 205 a.a., with molecular weight of ~34.0 kDa.

研究背景

Ephrin-B1/EFNB1 蛋白是一种细胞表面跨膜配体,与 Eph 受体相互作用,Eph 受体是受体酪氨酸激酶家族,对神经元、血管和上皮发育至关重要。这种结合导致相邻细胞之间依赖于接触的双向信号传导。Ephrin-B1/EFNB1 对受体酪氨酸激酶 EPHB1/ELK 显示出高亲和力,并且还可以结合 EPHB2EPHB3。体外研究表明,它可以诱导连合轴突和生长锥的塌陷,表明它在定向纵向突出轴突方面发挥着作用。Ephrin-B1/EFNB1 通过其 PDZ 结合基序与 GRIP1 和 GRIP2 以及 TLE1 相互作用。此外,Ephrin-B1/EFNB1 的胞内结构域肽与 ZHX2 相互作用,增强 ZHX2 的转录抑制活性。

种属

Human

表达系统

HEK293

标签

C-6*His

蛋白编号

P98172 (L28-G232)

基因 ID
蛋白结构
N-term
EFNB1 (L28-G232)
Accession # P98172
6*His
C-term
同用名
rHuEphrin-B1/EFNB1, His; Ephrin-B1; EFL-3; ELK ligand; EPH-related receptor tyrosine kinase ligand 2; LERK-2
氨基酸序列

LAKNLEPVSWSSLNPKFLSGKGLVIYPKIGDKLDIICPRAEAGRPYEYYKLYLVRPEQAAACSTVLDPNVLVTCNRPEQEIRFTIKFQEFSPNYMGLEFKKHHDYYITSTSNGSLEGLENREGGVCRTRTMKIIMKVGQDPNAVTPEQLTTSRPSKEADNTVKMATQAPGSRGSLGDSDGKHETVNQEEKSGPGASGGSSGDPDG

分子量

Approximately 34.0 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

Ephrin-B1/EFNB1 Protein, Human (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Ephrin-B1/EFNB1 Protein, Human (HEK293, His)
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HY-P70402
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