EPHX1 蛋白, Human (His-SUMO)

The EPHX1 protein is an important biotransformation enzyme that hydrolyzes aromatic hydrocarbons and aliphatic epoxides by catalyzing the transaddition of water. This makes the epoxide less reactive and more water-soluble. EPHX1 Protein, Human (His-SUMO) is the recombinant human-derived EPHX1 protein, expressed by E. coli , with N-6*His, N-SUMO labeled tag. The total length of EPHX1 Protein, Human (His-SUMO) is 455 a.a., with molecular weight of ~66 kDa.

EPHX1 蛋白是一种重要的生物转化酶，促进芳烃和脂肪族环氧化物的水解。通过水的反式加成，EPHX1 催化这种转化，使环氧化物的反应性降低，水溶性提高。除了在异生物质代谢中的作用外，EPHX1 在内源性脂质的代谢途径中发挥着重要作用，特别是在含环氧化物的脂肪酸的加工中。值得注意的是，EPHX1 参与丰富的内源性大麻素 2-花生四烯酰甘油 (2-AG) 的代谢，将其转化为游离花生四烯酸 (AA) 和甘油。这些酶活性凸显了 EPHX1 在调节各种底物的生物利用度和反应性方面的多功能作用，有助于重要的细胞过程和脂质代谢。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

N-6*His;N-SUMO

P07099 (M1-Q455)

2052  [NCBI]

MWLEILLTSVLGFAIYWFISRDKEETLPLEDGWWGPGTRSAAREDDSIRPFKVETSDEEIHDLHQRIDKFRFTPPLEDSCFHYGFNSNYLKKVISYWRNEFDWKKQVEILNRYPHFKTKIEGLDIHFIHVKPPQLPAGHTPKPLLMVHGWPGSFYEFYKIIPLLTDPKNHGLSDEHVFEVICPSIPGYGFSEASSKKGFNSVATARIFYKLMLRLGFQEFYIQGGDWGSLICTNMAQLVPSHVKGLHLNMALVLSNFSTLTLLLGQRFGRFLGLTERDVELLYPVKEKVFYSLMRESGYMHIQCTKPDTVGSALNDSPVGLAAYILEKFSTWTNTEFRYLEDGGLERKFSLDDLLTNVMLYWTTGTIISSQRFYKENLGQGWMTQKHERMKVYVPTGFSAFPFELLHTPEKWVRFKYPKLISYSYMVRGGHFAAFEEPELLAQDIRKFLSVLERQ

Approximately 66 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm solution of 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.