ER beta/ESR2 蛋白, Human (His)

ER beta/ESR2 Protein, a nuclear hormone receptor, binds estrogens akin to ESR1/ER-alpha. It activates estrogen-dependent reporter genes with ERE. However, it lacks ligand binding ability and exhibits minimal ERE binding, resulting in the loss of ligand-dependent transactivation ability. ER beta/ESR2 Protein, Human (His) is the recombinant human-derived ER beta/ESR2 protein, expressed by E. coli , with N-6*His labeled tag. The total length of ER beta/ESR2 Protein, Human (His) is 323 a.a., with molecular weight of 35-40 kDa.

ER beta/ESR2 是一种核激素受体，表现出与 ESR1/ER-α 相当的雌激素结合能力，反过来，以雌激素依赖性方式触发含有雌激素反应元件 (ERE) 的报告基因的表达。然而，值得注意的是，ER beta/ESR2缺乏内在的配体结合能力，并且表现出没有或极小的ERE结合活性，导致配体依赖性反式激活能力的减弱或丧失。这种双重性质，即它可以结合雌激素，但缺乏典型的配体依赖性激活潜力，强调了其独特的调节作用，并强调了它对雌激素介导的信号通路的复杂网络的贡献。

Human

E. coli

N-6*His

Q92731-3 (M1-A323)

2100  [NCBI]

MDIKNSPSSLNSPSSYNCSQSILPLEHGSIYIPSSYVDSHHEYPAMTFYSPAVMNYSIPSNVTNLEGGPGRQTTSPNVLWPTPGHLSPLVVHRQLSHLYAEPQKSPWCEARSLEHTLPVNRETLKRKVSGNRCASPVTGPGSKRDAHFCAVCSDYASGYHYGVWSCEGCKAFFKRSIQGHNDYICPATNQCTIDKNRRKSCQACRLRKCYEVGMVKCGSRRERCGYRLVRRQRSADEQLHCAGKAKRSGGHAPRVRELLLDALSPEQLVLTLLEAEPPHVLISRPSAPFTEASMMMSLTKLADKELVHMISWAKKIPGMRGNA

35-40 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCl, pH 8.0 or 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0 or PBS, 6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.